Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter
	Lin, Lu 1,2; Ji, Yuetong 1,2; Tu, Qichao 3,4; Huang, Ranran 1,2; Teng, Lin 1,2; Zeng, Xiaowei 1,2; Song, Houhui 1,2; Wang, Kun 1,2; Zhou, Qian 1,2; Li, Yifei 1,2
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2013-07-22
卷号	6 期号:1 页码:1-17
关键词	Shock Adaptation Ethanol Microevolution Thermophile
中文摘要	Introduction: The molecular links between shock-response and adaptation remain poorly understood, particularly for extremophiles. This has hindered rational engineering of solvent tolerance and correlated traits (e.g., productivity) in extremophiles. To untangle such molecular links, here we established a model that tracked the microevolution from shock to adaptation in thermophilic bacteria. Method: Temporal dynamics of genomes and transcriptomes was tracked for Thermoanaerobacter sp. X514 which under increasing exogenous ethanol evolved from ethanol-sensitive wild-type (Strain X) to tolerance of 2%- (XI) and eventually 6%-ethanol (XII). Based on the reconstructed transcriptional network underlying stress tolerance, genetic engineering was employed to improve ethanol tolerance and production in Thermoanaerobacter. Results: The spontaneous genome mutation rate (μg) of Thermoanaerobacter sp. X514, calculated at 0.045, suggested a higher mutation rate in thermophile than previously thought. Transcriptomic comparison revealed that shock-response and adaptation were distinct in nature, whereas the transcriptomes of XII resembled those of the extendedly shocked X. To respond to ethanol shock, X employed fructose-specific phosphotransferase system (PTS), Arginine Deiminase (ADI) pathway, alcohol dehydrogenase (Adh) and a distinct mechanism of V-type ATPase. As an adaptation to exogenous ethanol, XI mobilized resistance-nodulation-cell division (RND) efflux system and Adh, whereas XII, which produced higher ethanol than XI, employed ECF-type ϭ24, an alcohol catabolism operon and phase-specific heat-shock proteins (Hsps), modulated hexose/pentose-transport operon structure and reinforced membrane rigidity. Exploiting these findings, we further showed that ethanol productivity and tolerance can be improved simultaneously by overexpressing adh or ϭ24 in X. Conclusion: Our work revealed thermophilic-bacteria specific features of adaptive evolution and demonstrated a rational strategy to engineer co-evolving industrial traits. As improvements of shock-response, stress tolerance and productivity have been crucial aims in industrial applications employing thermophiles, our findings should be valuable not just to the production of ethanol but also to a wide variety of biofuels and biochemicals.
英文摘要	Introduction: The molecular links between shock-response and adaptation remain poorly understood, particularly for extremophiles. This has hindered rational engineering of solvent tolerance and correlated traits (e. g., productivity) in extremophiles. To untangle such molecular links, here we established a model that tracked the microevolution from shock to adaptation in thermophilic bacteria.
学科主题	功能基因组
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	ESCHERICHIA-COLI ; CLOSTRIDIUM-ACETOBUTYLICUM ; BUTANOL STRESS ; SACCHAROMYCES-CEREVISIAE ; TRANSCRIPTIONAL ANALYSIS ; ISOBUTANOL TOLERANCE ; ADAPTIVE EVOLUTION ; SOLVENT TOLERANCE ; GENOMIC ANALYSIS ; SIGMA-FACTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000323377300001
公开日期	2014-03-21
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1616]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, BioEnergy Genome Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA 4.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA
推荐引用方式 GB/T 7714	Lin, Lu,Ji, Yuetong,Tu, Qichao,et al. Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter[J]. BIOTECHNOLOGY FOR BIOFUELS,2013,6(1):1-17.
APA	Lin, Lu.,Ji, Yuetong.,Tu, Qichao.,Huang, Ranran.,Teng, Lin.,...&Xu, Jian.(2013).Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter.BIOTECHNOLOGY FOR BIOFUELS,6(1),1-17.
MLA	Lin, Lu,et al."Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter".BIOTECHNOLOGY FOR BIOFUELS 6.1(2013):1-17.