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	Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803
	Liu Lian; Hu HanHua; Gao Hong; Xu XuDong
刊名	CHINESE SCIENCE BULLETIN
	2013-12-01
卷号	58期号:36页码:4616-4621
关键词	Synechocystis glycogen synthesis phosphoglucomutase phosphomannomutase
ISSN号	1001-6538
通讯作者	Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.
英文摘要	Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis. In Synechocystis sp. PCC 6803, sll0726 is predicted to encode PGM (phosphoglucomutase), slr1334 is predicted to encode a PGM/PMM (phosphomannomutase) bifunction enzyme. In comparison to the wild type, a sll0726-null mutant showed 3.4% PGM activity but 45%-69% glycogen content. Down-regulation of slr1334, an essential gene, by using a copper regulated promoter further decreased the PGM activity in the sll0726::Km(r) P (petE) -slr1334 double mutant to 0.3% of the wild type level. However, the glycogen content was not further decreased in parallel. In vitro, recombinant Sll0726 or Slr1334 showed predicted enzyme activities. Our results indicate that a relatively high level of glycogen can be maintained in Synechocystis mutants with low levels of PGM activity. The high PGM activity in the cyanobacterium may be required for turnover of glycogen or synthesis of other polysaccharides or oligosaccharides.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	BLUE-GREEN-ALGA ; PSEUDOMONAS-AERUGINOSA ; PHOSPHOGLUCOSAMINE MUTASE ; SP PCC-6803 ; CYANOBACTERIA ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; BIOSYNTHESIS ; PURIFICATION ; ANABAENA
收录类别	SCI
资助信息	National Natural Science Foundation of China [30825003]
语种	英语
WOS记录号	WOS:000327482400014
公开日期	2014-01-24
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19788]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Liu Lian,Hu HanHua,Gao Hong,et al. Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803[J]. CHINESE SCIENCE BULLETIN,2013,58(36):4616-4621.
APA	Liu Lian,Hu HanHua,Gao Hong,&Xu XuDong.(2013).Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803.CHINESE SCIENCE BULLETIN,58(36),4616-4621.
MLA	Liu Lian,et al."Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp PCC6803".CHINESE SCIENCE BULLETIN 58.36(2013):4616-4621.

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