S-nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy

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	S-nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy
	Xie, AF ; Duan, SJ ; Zhang, ZB ; Chen, YX ; Xue, LH ; Yang, GZ
刊名	IMMUNOLOGY LETTERS
	2003
卷号	85 期号:3 页码:225
关键词	NITRIC-OXIDE FLOW-CYTOMETRY CELL-DEATH GLUTATHIONE EXPRESSION
ISSN号	0165-2478
通讯作者	Duan, SJ (reprint author), Chinese Acad Tradit Chinese Med, Guang An Men Hosp, Beijing 100053, Peoples R China.
中文摘要	This study is an attempt to deeply understand the mechanisms ensuring self-tolerance of T cells via clonal deletion of thymocytes and exploring T lymophocyte homeostasis by observing the apoptosis of single mouse thymocyte induced by S-nitrosoglutathione (GSNO, a nitric oxide donor) using fluorescence near-field scanning optical microscopy (NSOM) in illumination mode. The GSNO-induced thymocytes were stained with propidium iodide containing 0.01% Triton X-100 and excited with light of 488 nm and the emitting fluorescence at 525 nm. According to the NSOM fluorescence image and the simultaneously obtained topography image, the feature of mouse thymocyte apoptosis was characterized by scattering pattern of the fluorescence spots with the size 0.2-2.1 mum at the full width at half-maximum of fluorescence intensity 78-80 kHz in the GSNO-treated thymocyte nucleus. Whereas there is no fluorescence from the untreated thymocyte. The intensity of the fluorescence from the dexamethasone-treated thymocyte was much stronger than that from GSNO-induced thymocytes. Furthermore, the fluorescence distribution in the latter were concentrated in the nucleus. Those results also demonstrate the advantages of NSOM such as high spatial resolution and the topography of biology samples. (C) 2002 Elsevier Science B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/52715]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, AF,Duan, SJ,Zhang, ZB,et al. S-nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy[J]. IMMUNOLOGY LETTERS,2003,85(3):225.
APA	Xie, AF,Duan, SJ,Zhang, ZB,Chen, YX,Xue, LH,&Yang, GZ.(2003).S-nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy.IMMUNOLOGY LETTERS,85(3),225.
MLA	Xie, AF,et al."S-nitrosoglutathione-induced mouse thymocyte apoptosis studied by fluorescence near-field scanning optical microscopy".IMMUNOLOGY LETTERS 85.3(2003):225.