SAD phasing by OASIS-2004: case studies of dual-space fragment extension

CORC > 物理研究所 > 物理所公开发表论文 > 期刊论文

	SAD phasing by OASIS-2004: case studies of dual-space fragment extension
	Yao, DQ ; Huang, S ; Wang, JW ; Gu, YX ; Zheng, CD ; Fan, HF ; Watanabe, N ; Tanaka, I
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
	2006
卷号	62 页码:883
关键词	ANOMALOUS SCATTERING DATA CHROMIUM RADIATION PROTEIN REFINEMENT COMBINATION RESOLUTION
ISSN号	0907-4449
通讯作者	Fan, HF (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100080, Peoples R China.
中文摘要	The principle of dual- space phasing is used in dealing with protein SAD data. Four programs are involved in iterative dual- space fragment extension to improve automatic model building. OASIS- 2004 is used to break the phase ambiguity intrinsic in the SAD experiment. In the initial cycle, discrimination of SAD phase doublets is performed by the direct method incorporating the known anomalous- scattering substructure. In subsequent cycles, discrimination is performed by the direct method incorporating both the known anomalous- scattering substructure and the partial protein structure obtained from model building in the preceding cycle. DM is used to improve direct- method phases via density modification. RESOLVE is used for initial model building and ARP/ wARP is used to complete the structure. Case studies with three sets of difficult SAD data showed that the procedure is beneficial to high- throughput proteinstructure determination and all of the four programs involved make their unique contribution to the process.
收录类别	SCI
语种	英语
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/52288]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Yao, DQ,Huang, S,Wang, JW,et al. SAD phasing by OASIS-2004: case studies of dual-space fragment extension[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2006,62:883.
APA	Yao, DQ.,Huang, S.,Wang, JW.,Gu, YX.,Zheng, CD.,...&Tanaka, I.(2006).SAD phasing by OASIS-2004: case studies of dual-space fragment extension.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,62,883.
MLA	Yao, DQ,et al."SAD phasing by OASIS-2004: case studies of dual-space fragment extension".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 62(2006):883.