Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study

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	Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study
	Zhang, Tian 1; Li, Xi 1; Lu, Yang 1; Wu, Chunyun 1; Fang, Tingting 1; Liu, Peng 1; Zhang, Chaocan 2; Liang, Wei 3
刊名	BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
	2013-07-01
卷号	91 期号:1 页码:62-68
关键词	Metal Tetrahymena Microcalorimetry Toxicity Membrane
ISSN号	0007-4861
通讯作者	Li, X (reprint author), Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China.
中文摘要	The toxicity of Cr3+, Cu2+ and Cd2+ to Tetrahymena growth metabolism was studied by microcalorimetry at 28A degrees C, and the growth constant (k), peak time (T) and generation times (T (G)) were calculated. The metal ion concentrations that resulted in 50 % inhibition (IC (50)) of population growth were obtained through the dynamic parameters. The results indicated that the order of toxicity was Cd2+ > Cr3+ > Cu2+. Inductively coupled plasma-atomic emission spectrometry results suggested that the metal ions affected the permeability of the cell membrane. Observations of the Cd-exposed organisms by scanning electron microscopy revealed damage to the cell membrane in the form of an altered surface appearance. The cells suffered serious damage after sufficient acting time. Attenuated total reflection Fourier transform infrared spectra revealed that amide groups and PO2 (-) of the phospholipid phospho-diester, both located in the hydrophobic end of the outer layer of the cell membrane, were most readily affected.
英文摘要	The toxicity of Cr3+, Cu2+ and Cd2+ to Tetrahymena growth metabolism was studied by microcalorimetry at 28A degrees C, and the growth constant (k), peak time (T) and generation times (T (G)) were calculated. The metal ion concentrations that resulted in 50 % inhibition (IC (50)) of population growth were obtained through the dynamic parameters. The results indicated that the order of toxicity was Cd2+ > Cr3+ > Cu2+. Inductively coupled plasma-atomic emission spectrometry results suggested that the metal ions affected the permeability of the cell membrane. Observations of the Cd-exposed organisms by scanning electron microscopy revealed damage to the cell membrane in the form of an altered surface appearance. The cells suffered serious damage after sufficient acting time. Attenuated total reflection Fourier transform infrared spectra revealed that amide groups and PO2 (-) of the phospholipid phospho-diester, both located in the hydrophobic end of the outer layer of the cell membrane, were most readily affected.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	ACCUMULATION ; SOILS
收录类别	SCI
资助信息	Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003]
语种	英语
WOS记录号	WOS:000320881100011
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19598]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China 2.Wuhan Univ Technol, Sch Mat Sci & Engn, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Tian,Li, Xi,Lu, Yang,et al. Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study[J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,91(1):62-68.
APA	Zhang, Tian.,Li, Xi.,Lu, Yang.,Wu, Chunyun.,Fang, Tingting.,...&Liang, Wei.(2013).Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,91(1),62-68.
MLA	Zhang, Tian,et al."Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study".BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 91.1(2013):62-68.