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	Feasible plant regeneration in black pepper from petiole explants
	Ahmad, Nisar1,4; Guo, Bin2; Fazal, Hina3,5; Abbasi, Bilal Haider1; Liu, Chun-Zhao6; Mahmood, Tariq3; Shinwari, Zabta Khan1
刊名	JOURNAL OF MEDICINAL PLANTS RESEARCH
	2011-09-16
卷号	5期号:18页码:4590-4595
关键词	Piper nigrum callus organogenesis petiole 6-benzyladenine
ISSN号	1996-0875
其他题名	J. Med. Plants Res.
中文摘要	A feasible plant regeneration protocol was established for economically important plant, black pepper. Callogenesis was induced from petiole explants of potted plants incubated on murashige and skoog (MS) - medium supplemented with different concentrations of several phytohormones (PGRs). The best callus induction (85%) was observed for MS-medium supplemented with 0.5 mg l(-1) 6 - benzyladenine (BA) after 04 weeks of culture. Subsequent transfer of callus to MS medium containing similar PGRs induced shoot regeneration. Highest shoot regeneration (92%) was recorded for 0.5 mg l(-1) BA after 5 weeks of transfer. Furthermore, 8.1 shoots/explant were recorded for 0.5 mg l(-1) BA. Addition of 2.0 mg l(-1) of indole-3-butyric acid (IBA) produced 5.1 cm long shoots with 85% shoot organogenesis. Shoots produced healthy plantlets when transferred to MS medium containing several concentrations of indole butyric acid IBA. Regenerated plantlets were transferred to pots for acclimatization.
英文摘要	A feasible plant regeneration protocol was established for economically important plant, black pepper. Callogenesis was induced from petiole explants of potted plants incubated on murashige and skoog (MS) - medium supplemented with different concentrations of several phytohormones (PGRs). The best callus induction (85%) was observed for MS-medium supplemented with 0.5 mg l(-1) 6 - benzyladenine (BA) after 04 weeks of culture. Subsequent transfer of callus to MS medium containing similar PGRs induced shoot regeneration. Highest shoot regeneration (92%) was recorded for 0.5 mg l(-1) BA after 5 weeks of transfer. Furthermore, 8.1 shoots/explant were recorded for 0.5 mg l(-1) BA. Addition of 2.0 mg l(-1) of indole-3-butyric acid (IBA) produced 5.1 cm long shoots with 85% shoot organogenesis. Shoots produced healthy plantlets when transferred to MS medium containing several concentrations of indole butyric acid IBA. Regenerated plantlets were transferred to pots for acclimatization.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Chemistry, Medicinal
研究领域[WOS]	Pharmacology & Pharmacy
关键词[WOS]	PIPER-NIGRUM L. ; MEDICINAL-PLANT ; SOMATIC EMBRYOGENESIS ; SHOOT REGENERATION ; LEAF EXPLANTS ; ANTIOXIDANT ; CULTURES ; MICROPROPAGATION ; TISSUES ; SEEDS
收录类别	SCI
原文出处	://WOS:000299778500031
语种	英语
WOS记录号	WOS:000299778500031
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6346]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Quaid I Azam Univ, Fac Biol Sci, Dept Biotechnol, Islamabad 45320, Pakistan 2.NW Univ Xian, Sch Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China 3.Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad 45320, Pakistan 4.Nucl Inst Food & Agr, Tarnab Peshawar 2500, Pakistan 5.Pakistan Council Sci & Ind Res PCSIR Labs Complex, Peshawar 2500, Pakistan 6.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ahmad, Nisar,Guo, Bin,Fazal, Hina,et al. Feasible plant regeneration in black pepper from petiole explants[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH,2011,5(18):4590-4595.
APA	Ahmad, Nisar.,Guo, Bin.,Fazal, Hina.,Abbasi, Bilal Haider.,Liu, Chun-Zhao.,...&Shinwari, Zabta Khan.(2011).Feasible plant regeneration in black pepper from petiole explants.JOURNAL OF MEDICINAL PLANTS RESEARCH,5(18),4590-4595.
MLA	Ahmad, Nisar,et al."Feasible plant regeneration in black pepper from petiole explants".JOURNAL OF MEDICINAL PLANTS RESEARCH 5.18(2011):4590-4595.

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