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	EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES
	Liu, Yang1,2,3; Xu, Xia1,4; Ma, Xuehu3; Liu, Jing2; Cui, Zhanfeng1
刊名	CRYOLETTERS
	2011-09-01
卷号	32期号:5页码:425-435
关键词	cryopreservation mesenchymal stem cells well defined freezing solutions
ISSN号	0143-2044
其他题名	CryoLetters
中文摘要	The objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs.
英文摘要	The objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology ; Physiology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics ; Physiology
关键词[WOS]	BONE-MARROW ; VIABILITY ; PERMEABILITY ; TREHALOSE ; RECOVERY ; RABBIT ; TISSUE ; SPERM ; SIZE
收录类别	SCI
原文出处	://WOS:000296681200007
语种	英语
WOS记录号	WOS:000296681200007
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6312]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England 2.Dalian Med Univ, Affiliated Hosp 1, Sino UK Regenerat Med Ctr, Dalian 116011, Peoples R China 3.Dalian Univ Technol, Sch Chem Engn, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yang,Xu, Xia,Ma, Xuehu,et al. EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES[J]. CRYOLETTERS,2011,32(5):425-435.
APA	Liu, Yang,Xu, Xia,Ma, Xuehu,Liu, Jing,&Cui, Zhanfeng.(2011).EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES.CRYOLETTERS,32(5),425-435.
MLA	Liu, Yang,et al."EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES".CRYOLETTERS 32.5(2011):425-435.

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