A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization
	Tang, Ya-Jie 1,2; Xu, Xiao-Ling 1; Zhong, Jian-Jiang 3
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2010-02-01
卷号	33 期号:2 页码:237-246
关键词	Biotransformation process optimization 4 '-Demethylepipodophyllotoxin Bacillus fusiformis 4 '-Demethylepipodophyllic acid
ISSN号	1615-7591
通讯作者	Tang, YJ
英文摘要	This work optimized the novel biotransformation process of 4'-demethylepipodophyllotoxin (DMEP) into 4'-demethylepipodophyllic acid (DMEPA) by Bacillus fusiformis CICC 20463. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/L of yeast extract and 10 g/L of peptone were optimal for DMEPA production (i.e., 2.81 +/- A 0.21 mg/L), while not beneficial for the cell growth of B. fusiformis. This indicated that the biosynthesis of DMEPA was not corresponded well to the cell growth of B. fusiformis. 40 g/L of sucrose was optimal for DMEPA production (i.e., 2.94 +/- A 0.17 mg/L), and 3 g/L of NaCl was the best for DMEPA production (i.e., 4.10 +/- A 0.18 mg/L). Secondly, the production of DMEPA was significantly enhanced by the control of substrate concentration and culture pH. 100 mg/L of substrate was optimal for DMEPA production (i.e., 6.47 +/- A 0.35 mg/L), and DMEPA concentration was enhanced to 38.78 mg/L by controlling culture pH at 9.0 in the stirred-tank bioreactors. The fundamental information obtained in this study provides a simple and efficient way to produce DMEPA by biotransformation.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	CELL-SUSPENSION CULTURES ; PODOPHYLLOTOXIN CONGENERS ; BIOLOGICAL EVALUATION ; ANALOGS ; 4'-DEMETHYLEPIPODOPHYLLOTOXIN ; PICROPODOPHYLLIN ; BIOCATALYSIS ; DERIVATIVES ; LIGNANS ; AGENTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000273588200007
公开日期	2013-11-27
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6214]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Hubei Univ Technol, Coll Bioengn, Minist Educ, Key Lab Fermentat Engn,Hubei Prov Key Lab Ind Mic, Wuhan 430068, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Tang, Ya-Jie,Xu, Xiao-Ling,Zhong, Jian-Jiang. A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2010,33(2):237-246.
APA	Tang, Ya-Jie,Xu, Xiao-Ling,&Zhong, Jian-Jiang.(2010).A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization.BIOPROCESS AND BIOSYSTEMS ENGINEERING,33(2),237-246.
MLA	Tang, Ya-Jie,et al."A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization".BIOPROCESS AND BIOSYSTEMS ENGINEERING 33.2(2010):237-246.