Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 宁波所知识产出

	Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells
	Tatjana ; Hans C Arora1, Mark P Jensen2, Ye Yuan1, Aiguo Wu3, Stefan Vogt4, Tatjana Paunesku1,5,* and Gayle E Woloschak1,5
刊名	Cancer Research
	2012-02-27
期号	72 页码:769—778
通讯作者	Tatjana
合作状况	李雨桐
中文摘要	Resistance to anthracyclines and other chemotherapeutics due to P-glycoprotein (PGP)-mediated export is a frequent problem in cancer treatment. Here we report that iron oxide-titanium dioxide core-shell nanocomposites can serve as efficient carriers for doxorubicin to overcome this common mechanism of drug resistance in cancer cells.Doxorubicin nanocarriers (DNCs) increased effective drug uptake in drug-resistant ovarian cells. Mechanistically, doxorubicin bound to the TiO2 surface by a labile bond that was severed upon acidification within cell endosomes. Upon its release doxorubicin traversed the intracellular milieu and entered the cell nucleus by a route that evaded PGP-mediated drug export. Confocal and x-ray fluorescence microscopy with flow cytometry were used to demonstrate the ability of DNC to modulate transferrin uptake and distribution in cells. Increased transferrin uptake occurred through clathrin-mediated endocytosis, indicating that nanocomposites and DNCs may both interfere with removal of transferrin from cells. Together, our findings show that DNCs not only provide an alternative route of delivery of doxorubicin to PGP-over-expressing cancer cells, but may also boost the uptake of transferrin-tagged therapeutic agents.
学科主题	材料科学
原文出处	EI收录
公开日期	2013-12-16
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/9564]
专题	宁波材料技术与工程研究所_宁波所知识产出
推荐引用方式 GB/T 7714	Tatjana,Hans C Arora1, Mark P Jensen2, Ye Yuan1, Aiguo Wu3, Stefan Vogt4, Tatjana Paunesku1,5,* and Gayle E Woloschak1,5. Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells[J]. Cancer Research,2012(72):769—778.
APA	Tatjana,&Hans C Arora1, Mark P Jensen2, Ye Yuan1, Aiguo Wu3, Stefan Vogt4, Tatjana Paunesku1,5,* and Gayle E Woloschak1,5.(2012).Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells.Cancer Research(72),769—778.
MLA	Tatjana,et al."Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells".Cancer Research .72(2012):769—778.