Quantitative visualization of myocardial ischemia- reperfusion-induced cardiac lesions via ferroptosis

doi:10.7150/thno.89190

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Quantitative visualization of myocardial ischemia- reperfusion-induced cardiac lesions via ferroptosis
	Yang, Wenwen 1,2,3; Wang, Yueqi 3; Fu, Changgeng 2; Li, Changjian 4; Feng, Feng 1,5; Li, Hongzheng 1,2,6; Tan, Ling 1,2; Qu, Hua 1; Hui, Hui 3; Wang, Jingjing 3,8
刊名	THERANOSTICS
	2024
卷号	14 期号:3 页码:1081-1097
关键词	myocardial ischemia-reperfusion ferroptosis magnetic particle imaging quantitative visualization
ISSN号	1838-7640
DOI	10.7150/thno.89190
通讯作者	Wang, Jingjing(yueqi.wang@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Long, Linzi(qixiang830803@163.com)
英文摘要	Myocardial ischemia-reperfusion (MI/R) injury is a complication in vascular reperfusion therapy for MI, occurring in approximately 60% of patients. Ferroptosis is an important process in the development of MI/R cardiac lesions. Transferrin receptor 1 (TfR1), a marker of ferroptosis, corresponds to the changes in MI/R cardiac lesions and is expected to be a biomarker for detecting MI/R-induced ferroptosis. However, the noninvasive in vivo visualization of ferroptosis in MI/R is a big challenge. Thus, this study aimed to develop a novel multimodal imaging platform to identify markers of MI/R cardiac lesions in vivo through targeting TfR1. Methods: Magnetic particle imaging (MPI) modality for ferroptosis based on superparamagnetic cubic -iron oxide nanoparticles (SCIO NPs), named feMPI, has been developed. FeMPI used TfR1 as a typical biomarker. The feMPI probe (SCIO-ICG-CRT-CPPs NPs, CCI NPs) consists of SCIO NPs, TfR1-targeting peptides (CRT), cell -penetrating peptides (CPPs), and indocyanine green (ICG). The specificity and sensitivity of CCI NPs in the MI/R mouse model were evaluated by MPI, magnetic resonance imaging (MRI), and near -infrared (NIR) fluorescent imaging. Results: The intensity of the MPI signal correlates linearly with the percentage of infarct area in MI/R stained by TTC, enabling a quantitative assessment of the extent of cardiac lesions. Notably, these findings are consistent with the standard clinical biochemical indicators in MI/R within the first 24 h. FeMPI detects cardiac injury approximately 48 h prior to the current clinical imaging detection methods of MI/R. Conclusion: The feMPI strategy can be a powerful tool for studying the process of MI/R-induced ferroptosis in vivo, providing clues for molecular imaging and drug development of ferroptosis-related treatments.
资助项目	Science and Technology Innovation Project of China Academy of Chinese Medical Sciences[CI2021A05013] ; National Natural Science Foundation of China[92159303] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81930053] ; Beijing Science Fund[JQ22023] ; Beijing Natural Science Foundation[7212207] ; Project of High-Level Talents Team Introduction in Zhuhai City (Zhuhai)[HLHPTP201703]
WOS关键词	DELIVERY ; INJURY
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:001163065500024
资助机构	Science and Technology Innovation Project of China Academy of Chinese Medical Sciences ; National Natural Science Foundation of China ; Beijing Science Fund ; Beijing Natural Science Foundation ; Project of High-Level Talents Team Introduction in Zhuhai City (Zhuhai)
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/57870]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Wang, Jingjing; Tian, Jie; Long, Linzi
作者单位	1.China Acad Chinese Med Sci, Xiyuan Hosp, Dept Cardiol, Beijing 100091, Peoples R China 2.Natl Clin Res Ctr Cardiovasc Dis Tradit Chinese Me, Beijing 100091, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging,State Key Lab Manageme, Beijing 100190, Peoples R China 4.Beihang Univ, Sch Engn Med, Beijing 100191, Peoples R China 5.Zhejiang Univ, Coll Energy Engn, Hangzhou 310058, Zhejiang, Peoples R China 6.Beijing Univ Tradit Chinese Med, Grad Sch, Beijing 100029, Peoples R China 7.Gen Hosp Peoples Liberat Army China, Med Ctr 1, Dept Cardiovasc Med, Beijing 100853, Peoples R China 8.Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Peoples R China
推荐引用方式 GB/T 7714	Yang, Wenwen,Wang, Yueqi,Fu, Changgeng,et al. Quantitative visualization of myocardial ischemia- reperfusion-induced cardiac lesions via ferroptosis[J]. THERANOSTICS,2024,14(3):1081-1097.
APA	Yang, Wenwen.,Wang, Yueqi.,Fu, Changgeng.,Li, Changjian.,Feng, Feng.,...&Long, Linzi.(2024).Quantitative visualization of myocardial ischemia- reperfusion-induced cardiac lesions via ferroptosis.THERANOSTICS,14(3),1081-1097.
MLA	Yang, Wenwen,et al."Quantitative visualization of myocardial ischemia- reperfusion-induced cardiac lesions via ferroptosis".THERANOSTICS 14.3(2024):1081-1097.