Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2 ',4,4 '-tetrabromodiphenyl ether

	Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2 ',4,4 '-tetrabromodiphenyl ether
	Shi Pengju 1; Dong Shihang 1; Zhang Huanjian 2; Wang Peiliang 3; Niu Zhuang 1; Fang Yan 1
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2018
卷号	36 期号:2 页码:490-507
关键词	POLYBROMINATED DIPHENYL ETHERS BROMINATED FLAME RETARDANTS GENE-EXPRESSION MYTILUS-GALLOPROVINCIALIS RUDITAPES-PHILIPPINARUM AROMATIC-HYDROCARBONS ANTIOXIDANT RESPONSES DIALLYL PHTHALATE DISSOLVED-OXYGEN DIGESTIVE GLAND transcriptomic response Mactra veneriformis 2,2'4,4'-tetrabromodiphenyl ether
ISSN号	2096-5508
其他题名	Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2',4,4'-tetrabromodiphenyl ether
英文摘要	Polybrominated diphenyl ethers (PBDEs) are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However, the mechanism of its toxicity remains unclear. In this study, high-throughput sequencing was performed using the clam Mactra veneriformis, a good model for toxicological research, to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 mu g/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648, 154 225, and 124 985 unigenes by de novo assembly of the control group reads (CG), BDE-47 group reads (BDEG), and control and BDE-47 reads (CG & BDEG), respectively. We annotated 32 176 unigenes from the CG & BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover, 17 625 differentially expressed genes (DEGs) were detected, with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification, antioxidant defense, immune response, apoptosis, and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR, which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the M. veneriformis model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity.
语种	英语
CSCD记录号	CSCD:6290038
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/35111]
专题	中国科学院烟台海岸带研究所
作者单位	1.鲁东大学 2.中国科学院烟台海岸带研究所 3.Weihai Hongrun Marine Technology Co Ltd, Weihai 264200, Peoples R China
推荐引用方式 GB/T 7714	Shi Pengju,Dong Shihang,Zhang Huanjian,et al. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2 ',4,4 '-tetrabromodiphenyl ether[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):490-507.
APA	Shi Pengju,Dong Shihang,Zhang Huanjian,Wang Peiliang,Niu Zhuang,&Fang Yan.(2018).Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2 ',4,4 '-tetrabromodiphenyl ether.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),490-507.
MLA	Shi Pengju,et al."Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2 ',4,4 '-tetrabromodiphenyl ether".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):490-507.