A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)

doi:10.3390/app112210510

	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
	Wang, Xin; Wang, Guangxin; Xiao, Yuan; Zuo, Yanxia; Zhou, Fang
刊名	APPLIED SCIENCES-BASEL
	2021-11-01
卷号	11 期号:22 页码:16
关键词	micro-computed tomography zebrafish three-dimensional imaging fat content fat distribution
DOI	10.3390/app112210510
通讯作者	Zuo, Yanxia(yxzuo@ihb.ac.cn) ; Zhou, Fang(zhoufang@ihb.ac.cn)
英文摘要	Featured Application: The detection of fish body fat distribution and content is key in the study of lipid metabolism. Our study established an imaging technology for the rapid and nondestructive detection of the fat distribution and content of fish by micro-CT.Due to problems such as unbalanced intake of nutrients or excessive intake of energy, cultured fish accumulate fat in places such as the abdominal cavity, liver, and muscle, resulting in fatty liver, reducing the quality of fish meat, and even causing many fish deaths, resulting in losses to aquaculture production. Therefore, research on lipid metabolism in fish is important and has attracted increasing attention. The detection of fish body fat distribution and content is a key to such research. The existing methods for detecting fat distribution and content in fish have limitations, such as cumbersome procedures and damage to fish tissues, and thus, is imperative to develop a simple, fast, nondestructive fat detection technology. Taking zebrafish as the research material, this study established an imaging technology for the rapid and nondestructive detection of the fat distribution and content of fish by micro-computed tomography (micro-CT), optimized the fat CT-scanning method, determined the steps of fat quantitation in the CTAn data processing software, and constructed a three-dimensional (3D) model of zebrafish adipose tissue. This technology reveals the distribution of fish adipose tissue in an all-round way, and thus, it will play an important role in the study of lipid metabolism in fish.
WOS关键词	LIPID-METABOLISM ; FAT ; GROWTH ; MUSCLE ; RISK
WOS研究方向	Chemistry ; Engineering ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000726819300001
资助机构	Technical Innovation Project of Instrument and Equipment Function Development of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34780]
专题	中国科学院烟台海岸带研究所
通讯作者	Zuo, Yanxia; Zhou, Fang
作者单位	Chinese Acad Sci, Anal & Testing Ctr, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xin,Wang, Guangxin,Xiao, Yuan,et al. A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)[J]. APPLIED SCIENCES-BASEL,2021,11(22):16.
APA	Wang, Xin,Wang, Guangxin,Xiao, Yuan,Zuo, Yanxia,&Zhou, Fang.(2021).A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT).APPLIED SCIENCES-BASEL,11(22),16.
MLA	Wang, Xin,et al."A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)".APPLIED SCIENCES-BASEL 11.22(2021):16.