Oxidative stress biomarkers, physiological responses and proteomic profiling in oyster (Crassostrea gigas) exposed to microplastics with irregular-shaped PE and PET microplastic

doi:10.1016/j.scitotenv.2021.147425

	Oxidative stress biomarkers, physiological responses and proteomic profiling in oyster (Crassostrea gigas) exposed to microplastics with irregular-shaped PE and PET microplastic
	Teng, Jia 1,2,3,4; Zhao, Jianmin 1,2,3; Zhu, Xiaopeng 1,2,3,4; Shan, Encui 1,2,3,4; Wang, Qing 1,2,3
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2021-09-10
卷号	786 页码:14
关键词	Microplastics Physiological response iTRAQ Lipid metabolism Molecular mechanism
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2021.147425
通讯作者	Wang, Qing(qingwang@yic.ac.cn)
英文摘要	Microplastics (MPs) are currently one of the major environmental problems due to the large amount released in aquatic and terrestrial ecosystems as well as their potential impacts on organisms and human health. However, the molecular mechanisms underlying MP toxicity remain elusive and insufficiently understood. In this study, oysters were exposed to irregular MPs composed of polyethylene and polyethylene terephthalate at concentrations of 10 and 1000 mu g L & minus;1 for 21 days under static conditions, and physiological responses and changes in antioxidant enzymatic activity were investigated. Moreover, the protein expression profiles in the digestive glands were analyzed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method. And the expression of ten potentially regulated genes was analyzed by qRT-PCR in different tissues under MP exposure. It was demonstrated that PE and PET MP exposure had no distinct adverse effects on physiological rates, while inducing oxidative stresses in the oyster. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of DEPs revealed that MP exposure mainly disturbed the & ldquo;arachidonic acid metabolism & rdquo;, & ldquo;linoleic acid metabolism & rdquo; and & ldquo;glycerophospholipid metabolism & rdquo; processes in oysters. Gene Ontology enrichment analysis revealed that MPs affected the oxidation-reduction process, lipid metabolism process and pentose phosphate pathway. Moreover, a significant increase in the mRNA expression of genes related to lipid and aerobic metabolism and apoptosis pathways was observed after MP exposure. In summary, our results indicated that MPs could induce oxidative stress and alter lipid and glucose metabolism processes in oysters. Additionally, the weight of evidence (WOE) model analysis suggested that high concentrations of MPs were more hazardous than low concentrations and typically higher for PET than PE. This study provided the effects of PE and PET MP toxicity on oysters at the molecular level, which will contribute to better understanding the defense mechanisms of oysters against MP exposure. (c) 2021 Elsevier B.V. All rights reserved. "glycerophospholipid metabolism" processes in oysters. Gene Ontology enrichment analysis revealed that MPs affected the oxidation-reduction process, lipid metabolism process and pentose phosphate pathway. Moreover, a significant increase in the mRNA expression of genes related to lipid and aerobic metabolism and apoptosis pathways was observed after MP exposure. In summary, our results indicated that MPs could induce oxidative stress and alter lipid and glucose metabolism processes in oysters. Additionally, the weight of evidence (WOE) model analysis suggested that high concentrations of MPs were more hazardous than low concentrations and typically higher for PET than PE. This study provided the effects of PE and PET MP toxicity on oysters at the molecular level, which will contribute to better understanding the defense mechanisms of oysters against MP
WOS关键词	MYTILUS-EDULIS L. ; POLYSTYRENE MICROPLASTICS ; METABOLIC-RESPONSES ; BOHAI SEA ; PROTEIN ; TEMPERATURE ; INGESTION ; TOXICITY ; POLLUTION ; ABUNDANCE
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000660250500002
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34764]
专题	中国科学院烟台海岸带研究所
通讯作者	Wang, Qing
作者单位	1.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China 4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Teng, Jia,Zhao, Jianmin,Zhu, Xiaopeng,et al. Oxidative stress biomarkers, physiological responses and proteomic profiling in oyster (Crassostrea gigas) exposed to microplastics with irregular-shaped PE and PET microplastic[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,786:14.
APA	Teng, Jia,Zhao, Jianmin,Zhu, Xiaopeng,Shan, Encui,&Wang, Qing.(2021).Oxidative stress biomarkers, physiological responses and proteomic profiling in oyster (Crassostrea gigas) exposed to microplastics with irregular-shaped PE and PET microplastic.SCIENCE OF THE TOTAL ENVIRONMENT,786,14.
MLA	Teng, Jia,et al."Oxidative stress biomarkers, physiological responses and proteomic profiling in oyster (Crassostrea gigas) exposed to microplastics with irregular-shaped PE and PET microplastic".SCIENCE OF THE TOTAL ENVIRONMENT 786(2021):14.