Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer

doi:10.1016/j.lwt.2023.115464

CORC > 海洋研究所 > 中国科学院海洋研究所 > 深海极端环境与生命过程研究中心

	Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer
	Song, Wen 1,2,3,4; Xing, Ronge 1,2,3; Yang, Haoyue 1,2; Gao, Kun 1,2; Liu, Song 1,2,3; Li, Xin 1,2; Yu, Huahua 1,2,3; Li, Pengcheng 1,2,3
刊名	LWT-FOOD SCIENCE AND TECHNOLOGY
	2023-10-01
卷号	188 页码:13
关键词	Eumelanin Enzyme -assisted optimization Squid ink Tetramer Antioxidant activity
ISSN号	0023-6438
DOI	10.1016/j.lwt.2023.115464
通讯作者	Xing, Ronge(xingronge@qdio.ac.cn)
英文摘要	The investigation into natural eumelanin found in squid ink is an emerging research topic due to its antioxidative properties. However, the methodologies for acquiring pure eumelanin and establishing the intricate relationship between its structure and antioxidative efficacy remain limited. Novel enzyme extraction technique, termed response surface optimization, was employed to synthesize high-purity eumelanin, and its antioxidant activity was evaluated at free radical scavenging and cellular levels and correlated with detailed tetrameric structure prediction for the first time. Results demonstrated that enzymatic extraction technique yielded eumelanin (ANF) nanostructure with stability, boasting particle size of 112.35 nm, a maximum absorption wavelength of 224 nm, a Delta Hpp value of 0.4802 mT, and a purity of 92%. Compared with melanin standard (MelS), ANF had superior free radical scavenging activity, eliminating 89.62% of DPPH radicals, 96.79% of superoxide ion radicals, and 93.20% of hydroxyl radicals, and better repair of cellular oxidative damage. Based on molecular energy, molecular electrostatic potential (MEP), and molecular orbitals by Gaussian calculations, the tetrameric structure (DHICA3-DHI1"' and "DHICA3-DHI1") with significant antioxidant activity contained inside eumelanin was anticipated. Our findings reveal its potential as a natural antioxidant, offering broad prospects for antioxidant food additives or therapeutic applications in nutritional and pharmaceutical ingredients.
资助项目	Shandong Key R D Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Fujian Science and Technology Planning Project -STS Program[2021T3013]
WOS关键词	MELANIN ; PROTEINS ; BEHAVIOR ; PIGMENT ; VIVO
WOS研究方向	Food Science & Technology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001109520500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/183954]
专题	海洋研究所_深海极端环境与生命过程研究中心
通讯作者	Xing, Ronge
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100000, Peoples R China
推荐引用方式 GB/T 7714	Song, Wen,Xing, Ronge,Yang, Haoyue,et al. Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2023,188:13.
APA	Song, Wen.,Xing, Ronge.,Yang, Haoyue.,Gao, Kun.,Liu, Song.,...&Li, Pengcheng.(2023).Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer.LWT-FOOD SCIENCE AND TECHNOLOGY,188,13.
MLA	Song, Wen,et al."Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer".LWT-FOOD SCIENCE AND TECHNOLOGY 188(2023):13.