Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90

doi:10.1016/j.cej.2024.148590

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	Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90
	Wang, Yingwen 2,3; Qi, Peng 1,2; Zhang, Dun 1,2; Zeng, Yan 1,2; Sun, Yan 1,2; Wang, Peng 1,2
刊名	CHEMICAL ENGINEERING JOURNAL
	2024-02-01
卷号	481 页码:9
关键词	Paper-based ATP assay Degradation of high viscosity hydrogel Nanozyme reactivation Onsite detection ZIF-90
ISSN号	1385-8947
DOI	10.1016/j.cej.2024.148590
通讯作者	Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) ; Wang, Peng(wangpeng@qdio.ac.cn)
英文摘要	Adenosine triphosphate (ATP) is an important biological biomarker, but its rapid extracellular decomposition poses challenges for on-site detection. We report a paper-based dual-readout viscosity flow sensor enabling rapid, equipment-free quantification of ATP. The assay integrates molybdenum disulfide nanozymes for signal amplification with zeolitic imidazolate framework-90 (ZIF-90) for selective ATP recognition and nanozyme regulation. In the presence of ATP, the intact chitosanase-encapsulated ZIF-90 structure was disintegrated to release chitosanase and thus hydrolyzed chitosan hydrogel, decreasing the viscosity of chitosan solution. Moreover, elimination of ZIF-90 inhibitory effect reactivated the catalytic activity of MoS2 nanozyme, resulting in the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to produce obvious color change. The analytical signals, combining the distance- and colorimetric- readout, were directly displayed on filter paper without further processed. The proposed dual-readout viscosity flow sensor exhibited remarkable sensitivity for ATP detection with detection limits down to 0.038 nM with the distance-readout and 0.057 nM for the colorimetric-readout. Furthermore, we developed an integrated paper kit with a printed scale and color card for standardized on-site analysis without instrumentation. Successful application for ATP quantification in bacterial biofilms and blood samples demonstrates the sensor's strong performance and potential for decentralized testing.
资助项目	National Natural Science Foundation of China[42376208]
WOS关键词	PATHOGENS ; ANTIBODY ; ACID
WOS研究方向	Engineering
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:001161457900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184441]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Qi, Peng; Zhang, Dun; Wang, Peng
作者单位	1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 3.Chinese Acad Agr Sci, Key Lab Tobacco Pest Monitoring & Integrated Manag, Tobacco Res Inst, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yingwen,Qi, Peng,Zhang, Dun,et al. Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90[J]. CHEMICAL ENGINEERING JOURNAL,2024,481:9.
APA	Wang, Yingwen,Qi, Peng,Zhang, Dun,Zeng, Yan,Sun, Yan,&Wang, Peng.(2024).Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90.CHEMICAL ENGINEERING JOURNAL,481,9.
MLA	Wang, Yingwen,et al."Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90".CHEMICAL ENGINEERING JOURNAL 481(2024):9.