Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation | |
Song, Weijia1,2,3,4; Song, Xiuxian1,2,3,4; Chi, Lianbao1,2,4; Zhu, Jianan1,2,4; Cao, Xihua1,2,3,4; Yu, Zhiming1,2,3,4 | |
刊名 | HARMFUL ALGAE |
2023-11-01 | |
卷号 | 129页码:8 |
关键词 | Alexandrium pacificum Extracellular toxins Toxin cell quota Growth phases |
ISSN号 | 1568-9883 |
DOI | 10.1016/j.hal.2023.102516 |
通讯作者 | Song, Xiuxian(songxx@qdio.ac.cn) |
英文摘要 | Paralytic shellfish toxins (PSTs) are widely distributed globally and are produced by Alexandrium pacificum in marine system. However, the characteristics of toxins producing and secreting associated with growth phases are still unclear, especially whether A. pacificum has the ability to actively secrete PSTs is controversial. In this study, variation characteristics of intracellular and extracellular PSTs contents associated with A. pacificum growth phases were investigated thoroughly. The results showed that intracellular and extracellular PSTs contents increased sharply during the exponential phase. But during the stationary phase, the intracellular PSTs content increased by only 26 %, and the extracellular PSTs content did not increase significantly. Since the increase in extracellular PSTs content mainly occurred at the exponential phase, when most cells were living, we speculated that active PSTs secretion of living cells might be an important production pathway of extracellular toxins besides leakage from dead cells. Furthermore, toxin cell quota variation associated with the growth phase was analysed. In the exponential phase, the toxin cell quota first increased and then decreased, with a maximum of 19.02 +/- 1.80 fmol/cell at 6 d. However, after entering the stationary phase, this value slowly increased again, suggesting that vigilance should be raised for the plateau of Alexandrium blooms. In addition, cells in the exponential phase mainly produced O-sulfated components such as GTX1&4, cells in the stationary phase mainly produced O-sulfate-free components such as GTX5. In this study, the toxigenic rules of A. pacificum were comprehensively uncovered, which provided theoretical guidance for the prevention and mitigation of A. pacificum blooms. |
资助项目 | Intergovernmental Innovation Coop-eration Project of the Ministry of Science and Technology[2022YFE0136400] ; National Natural Science Foundation of China[41976148] ; National Natural Science Foundation of China[42276220] |
WOS关键词 | SAXITOXIN ; DINOFLAGELLATE ; TEMPERATURE ; CULTURES ; CYANOBACTERIA ; BIOSYNTHESIS ; PURIFICATION ; DINOPHYCEAE ; KINETICS |
WOS研究方向 | Marine & Freshwater Biology |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:001097652800001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/183834] |
专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
通讯作者 | Song, Xiuxian |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Weijia,Song, Xiuxian,Chi, Lianbao,et al. Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation[J]. HARMFUL ALGAE,2023,129:8. |
APA | Song, Weijia,Song, Xiuxian,Chi, Lianbao,Zhu, Jianan,Cao, Xihua,&Yu, Zhiming.(2023).Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation.HARMFUL ALGAE,129,8. |
MLA | Song, Weijia,et al."Novel insights into toxin changes associated with the growth of Alexandrium pacificum: Revealing active toxin-secretion ability and toxin cell quota variation".HARMFUL ALGAE 129(2023):8. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论