LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

doi:10.24272/j.issn.2095-8137.2022.520

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus
	Ning, Xian-Hui 2,3; Han, Bing 2; Peng, Ye 2; Yin, Shao-Wu 1,2
刊名	ZOOLOGICAL RESEARCH
	2024-01-18
卷号	45 期号:1 页码:25-35
关键词	LncRNA SARM miRNA ceRNA Antibacterial response
ISSN号	2095-8137
DOI	10.24272/j.issn.2095-8137.2022.520
通讯作者	Yin, Shao-Wu(yinshaowu@163.com)
英文摘要	Long non-coding RNAs (lncRNAs) function as key modulators in mammalian immunity, particularly due to their involvement in lncRNA-mediated competitive endogenous RNA (ceRNA) crosstalk. Despite their recognized significance in mammals, research on lncRNAs in lower vertebrates remains limited. In the present study, we characterized the first immune-related lncRNA (pol-lnc78) in the teleost Japanese flounder (Paralichthys olivaceus). Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein (SARM), the fifth discovered member of the Toll/interleukin 1 (IL-1) receptor (TIR) adaptor family. This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor (TLR) signaling pathway. Specifically, SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1 beta and tumor necrosis factor-alpha (TNF-alpha). Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3' untranslated region (UTR), thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination. Furthermore, pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression. During infection, the negative regulators pol-lnc78 and SARM were significantly down-regulated, while pol-miR-n199-3p was significantly up-regulated, thus favoring host antibacterial defense. These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.
资助项目	National Natural Science Foundation of China[42006082] ; Natural Science Foundation of Jiangsu Province of China[BK20221323] ; JBGS Project of Seed Industry Revitalization in Jiangsu Province[JBGS [2021] 034] ; State Key Laboratory of Developmental Biology of Freshwater Fish[2021KF009]
WOS关键词	LONG NONCODING RNA ; VIBRIO-ANGUILLARUM ; MOLECULAR CHARACTERIZATION ; CAENORHABDITIS-ELEGANS ; TRANSCRIPTOME ANALYSIS ; IMMUNE-RESPONSE ; INNATE IMMUNITY ; MESSENGER-RNA ; PROTEIN ; MIRNAS
WOS研究方向	Zoology
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:001165342100002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184601]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yin, Shao-Wu
作者单位	1.Coinnovat Ctr Marine Bioind Technol Jiangsu Prov, Lianyungang 222005, Jiangsu, Peoples R China 2.Nanjing Normal Univ, Coll Marine Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ning, Xian-Hui,Han, Bing,Peng, Ye,et al. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus[J]. ZOOLOGICAL RESEARCH,2024,45(1):25-35.
APA	Ning, Xian-Hui,Han, Bing,Peng, Ye,&Yin, Shao-Wu.(2024).LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus.ZOOLOGICAL RESEARCH,45(1),25-35.
MLA	Ning, Xian-Hui,et al."LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus".ZOOLOGICAL RESEARCH 45.1(2024):25-35.