Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

doi:10.1016/j.jhazmat.2023.133147

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole
	Zhang, Weikang 1,2; Lin, Yingwu 4; Meng, Xiangmin 2; Wang, Qiaoning 3; Chen, Lingxin 3; Xu, Jiakun 1
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2024-03-05
卷号	465 页码:10
关键词	Sulfonamides Myoglobin Biocatalysis Biodegradation Directed evolution
ISSN号	0304-3894
DOI	10.1016/j.jhazmat.2023.133147
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn) ; Xu, Jiakun(xujk@ysfri.ac.cn)
英文摘要	Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation
WOS关键词	COMPOUND-I ; ANTIBIOTICS ; SULFAMETHOXAZOLE ; SULFONAMIDES ; KINETICS ; BIOTRANSFORMATION ; SULFAMETHAZINE ; TRANSFORMATION ; DEGRADATION ; ENVIRONMENT
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:001134426400001
资助机构	National Key R & D Program of China ; National Natural Science Foundation of China ; Central Public-Interest Scientific Institution Basal Research Fund, CAFS
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/33117]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, Lingxin; Xu, Jiakun
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,et al. Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,465:10.
APA	Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,Wang, Qiaoning,Chen, Lingxin,&Xu, Jiakun.(2024).Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole.JOURNAL OF HAZARDOUS MATERIALS,465,10.
MLA	Zhang, Weikang,et al."Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole".JOURNAL OF HAZARDOUS MATERIALS 465(2024):10.