Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure

doi:10.3390/microorganisms11061362

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	Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure
	Bao, Xu-Chong 3,4,5; Tang, Hong-Zhi 4,5; Li, Xue-Gong 2,5; Li, An-Qi 4,5; Qi, Xiao-Qing 2,5; Li, Deng-Hui 1,2; Liu, Shan-Shan 1,2; Wu, Long-Fei 6; Zhang, Wei-Jia 2,5
刊名	MICROORGANISMS
	2023-06-01
卷号	11 期号:6 页码:14
关键词	deep-sea bacterium bioluminescence high hydrostatic pressure oxidative stress reactive oxygen species
DOI	10.3390/microorganisms11061362
通讯作者	Wu, Long-Fei(wu@imm.cnrs.fr) ; Zhang, Wei-Jia(wzhang@idsse.ac.cn)
英文摘要	Bioluminescence is a common phenomenon in nature, especially in the deep ocean. The physiological role of bacterial bioluminescence involves protection against oxidative and UV stresses. Yet, it remains unclear if bioluminescence contributes to deep-sea bacterial adaptation to high hydrostatic pressure (HHP). In this study, we constructed a non-luminescent mutant of & UDelta;luxA and its complementary strain c-& UDelta;luxA of Photobacterium phosphoreum ANT-2200, a deep-sea piezophilic bioluminescent bacterium. The wild-type strain, mutant and complementary strain were compared from aspects of pressure tolerance, intracellular reactive oxygen species (ROS) level and expression of ROS-scavenging enzymes. The results showed that, despite similar growth profiles, HHP induced the accumulation of intracellular ROS and up-regulated the expression of ROS-scavenging enzymes such as dyp, katE and katG, specifically in the non-luminescent mutant. Collectively, our results suggested that bioluminescence functions as the primary antioxidant system in strain ANT-2200, in addition to the well-known ROS-scavenging enzymes. Bioluminescence contributes to bacterial adaptation to the deep-sea environment by coping with oxidative stress generated from HHP. These results further expanded our understanding of the physiological significance of bioluminescence as well as a novel strategy for microbial adaptation to a deep-sea environment.
WOS关键词	OXYGEN-CONSUMPTION ; VIBRIO-FISCHERI ; DNA ; STIMULATION ; REPAIR ; MECHANISMS ; LUCIFERASE ; SEQUENCE ; LIFE
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:001017173300001
资助机构	Key Research and Development Program of Hainan Province ; National Natural Science Foundation of China ; Sanya city
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/33336]
专题	烟台海岸带研究所_中科院烟台海岸带研究所知识产出
通讯作者	Wu, Long-Fei; Zhang, Wei-Jia
作者单位	1.BGI Qingdao, BGI Shenzhen, Qingdao 266555, Peoples R China 2.Inst Deep Sea Life Sci, Hainan Deep Sea Technol Lab, IDSSE BGI, Sanya 572000, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 5.Chinese Acad Sci, Inst Deep Sea Sci Engn, Lab Deep Sea Microbial Cell Biol, Sanya 572000, Peoples R China 6.Aix Marseille Univ, LCB, IMM, CNRS, F-13402 Marseille, France
推荐引用方式 GB/T 7714	Bao, Xu-Chong,Tang, Hong-Zhi,Li, Xue-Gong,et al. Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure[J]. MICROORGANISMS,2023,11(6):14.
APA	Bao, Xu-Chong.,Tang, Hong-Zhi.,Li, Xue-Gong.,Li, An-Qi.,Qi, Xiao-Qing.,...&Zhang, Wei-Jia.(2023).Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure.MICROORGANISMS,11(6),14.
MLA	Bao, Xu-Chong,et al."Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic Pressure".MICROORGANISMS 11.6(2023):14.