A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth | |
Xing, Mengyang1; Li, Hongli; Liu, Gangshuai; Zhu, Benzhong; Zhu, Hongliang; Grierson, Donald3,4; Luo, Yunbo; Fu, Daqi | |
刊名 | PLANT SCIENCE |
2022 | |
卷号 | 322 |
关键词 | Plant hormones Sepal growth Tomato SlMADS1 SlMC Transcription factors |
ISSN号 | 0168-9452 |
DOI | 10.1016/j.plantsci.2022.111366 |
文献子类 | Article |
英文摘要 | In flowering plants, sepals play important roles in the development of flowers and fruit, and both processes are regulated by MADS-box (MADS) transcription factors (TFs). SlMADS1 was previously reported to act as a negative regulator of fruit ripening. In this study, expression analysis shown that its transcripts were very highly expressed during the development of sepals. To test the role of SlMADS1, we generated KO-SlMADS1 (knock-out) tomato mutants by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) technology and over-expression of SlMADS1 (OE-SlMADS1). The sepals and individual cells of KO-SlMADS1 mutants were significantly elongated, compared with the wild type (WT), whereas the sepals of OE-SlMADS1 tomatoes were significantly shorter and their cells were wider. RNA-seq (RNA-sequencing) of sepal samples showed that ethylene-, gibberellin-, auxin-, cytokinin-and cell wall metabolism-related genes were significantly affected in both KO-SlMADS1 and OE-SlMADS1 plants with altered sepal size. Since SlMA-CROCALYX (MC) is known to regulate the development of tomato sepals, we also studied the relationship be-tween SlMC and SlMADS1 and the result showed that SlMADS1 interacts directly with SlMC. In addition, we also found that manipulating SlMADS1 expression alters the development of tomato plant leaves, roots and plant height. These results enrich our understanding of sepal development and the function of SlMADS1 throughout the plant. |
学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
电子版国际标准刊号 | 1873-2259 |
出版地 | CLARE |
WOS关键词 | ORGAN IDENTITY ; ETHYLENE ; AUXIN ; GENE ; XYLOGLUCAN ; EXPRESSION ; FLOWER ; BIOSYNTHESIS ; MACROCALYX ; COMPLEXES |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | ELSEVIER IRELAND LTD |
WOS记录号 | WOS:000830264800007 |
资助机构 | National Natural Science Foundation of China [NSFC 31571898, 32072277] |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29027] |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Fruit Biol, Beijing 100083, Peoples R China 2.Univ Nottingham, Sch Biosci, Plant Sci Div, Sutton Bonington Campus, Loughborough LE12 5RD, England 3.Zhejiang Univ, Lab Fruit Qual Biol, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Peoples R China 4.Chinese Acad Sci, Inst Bot, Innovat Acad Seed Design, Key Lab Plant Resources, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Xing, Mengyang,Li, Hongli,Liu, Gangshuai,et al. A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth[J]. PLANT SCIENCE,2022,322. |
APA | Xing, Mengyang.,Li, Hongli.,Liu, Gangshuai.,Zhu, Benzhong.,Zhu, Hongliang.,...&Fu, Daqi.(2022).A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth.PLANT SCIENCE,322. |
MLA | Xing, Mengyang,et al."A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth".PLANT SCIENCE 322(2022). |
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