New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS

doi:10.1016/j.aca.2022.339891

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	New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS
	Sun, Shanshan 4; Wang, Shujuan 3; Li, Shanshan 4; Wei, Liping 3; Wang, Xiaohan 4; Wang, Yizhou 4; Li, Bing 4; Hu, Yang 1; Wang, Liangsheng 4
刊名	ANALYTICA CHIMICA ACTA
	2022
卷号	1212
关键词	Phytosterol Paeonia ostii 'Fengdan' Pollen Anther wall Gas chromatography-mass spectrometry (GC-MS)
ISSN号	0003-2670
DOI	10.1016/j.aca.2022.339891
文献子类	Article
英文摘要	Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') by gas chromatography-mass spectrometry. The result indicated that the contents of phytosterols in pollen (3390.02 mg/100 g DW) and anther wall (929.70 mg/100 g DW) of P. ostii 'Fengdan' were relatively high. Meanwhile, eleven phytosterols were identified, among which five phytosterols were first identified in tree peony pollen. Moreover, the effects of wall breaking and different developmental stages on phytosterol contents of pollen and anther wall of P. ostii 'Fengdan' were also discussed. The result showed that wall breaking was beneficial to sterol detection of pollen, but not necessary for anther wall. For the best harvest time, the stage of S2 had the highest content of phytosterols. In conclusion, this study successfully establishes an analytical method for phytosterols by gas chromatography-mass spectrometry, and lays a foundation for the development and utilization of pollen and anther wall of P. ostii 'Fengdan' in functional foods and pharmaceutical industry.
学科主题	Chemistry, Analytical
电子版国际标准刊号	1873-4324
出版地	AMSTERDAM
WOS关键词	GAS-CHROMATOGRAPHY ; BEE POLLEN ; OILS ; PHYTOSTANOLS ; EXTRACTION ; FRACTION ; ESTERS ; FOODS ; SEEDS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000830632800001
资助机构	National Natural Science Foundation of China [32030095] ; Science and Technology Project of Lu'an Group [2019C062]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28971]
专题	中科院北方资源植物重点实验室
作者单位	1.Shennong Zhihua Biotechnol Shanxi Co Ltd, Changzhi 046011, Peoples R China 2.Inner Mongolia Agr Univ, Coll Desert Management, Hohhot 010018, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Sun, Shanshan,Wang, Shujuan,Li, Shanshan,et al. New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS[J]. ANALYTICA CHIMICA ACTA,2022,1212.
APA	Sun, Shanshan.,Wang, Shujuan.,Li, Shanshan.,Wei, Liping.,Wang, Xiaohan.,...&Wang, Liangsheng.(2022).New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS.ANALYTICA CHIMICA ACTA,1212.
MLA	Sun, Shanshan,et al."New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS".ANALYTICA CHIMICA ACTA 1212(2022).