ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana

doi:10.1007/s00425-022-03936-w

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	ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana
	Jiang, Wenbo ; Xia, Yaying 1,2,4; Su, Xiaojia 2,4; Pang, Yongzhen
刊名	PLANTA
	2022
卷号	256 期号:2
关键词	Arabidopsis thaliana ARF2 Flavonols MYB12 Proanthocyanidins TT2
ISSN号	0032-0935
DOI	10.1007/s00425-022-03936-w
文献子类	Article
英文摘要	Main conclusion Auxin response factor 2 acts as a positive regulator to fine-tune the spatial and temporal accumulation of flavonoid compounds, mainly flavonols and proanthocyanidins in Arabidopsis. Auxin response factor (ARF) proteins are reported to involve in auxin-mediated regulation of flavonoid biosynthesis. However, the detailed regulation mechanism of ARF remains still unknown. Here, we provide genetic and molecular evidence that one of the twenty-three ARF members-ARF2-positively regulates flavonoid biosynthesis at multi-level in tissue-specific manner in Arabidopsis thaliana. Loss-of-function mutation of ARF2 led to significant reduction in flavonoid content (e.g., flavonols and proanthocyanidins) in the seedlings and seeds of the Arabidopsis arf2 mutants. Over-expression of ARF2 increased flavonols and proanthocyanidins content in Arabidopsis. Additionally, the changes of flavonoid content correlate well with the transcript abundance of several regulatory genes (e.g., MYB11, MYB12, MYB111, TT2, and GL3), and key biosynthetic genes (e.g., CHS, F3'H, FLS, ANS, ANR, TT12, TT19, and TT15), in the arf2 mutant and ARF2 over-expression lines. Transient transactivation assays with site-directed mutagenesis confirmed that ARF2 directly regulates the expression of MYB12 and FLS genes in the flavonol pathway and ANR in the proanthocyanidin pathway, and indirectly regulates MYB11 and MYB111 genes in the flavonol pathway, and ANS, TT12, TT19 and TT15 genes in the proanthocyanidin pathway. Further genetic results indicated that ARF2 acts upstream of MYB12 to regulate flavonol accumulation, and of TT2 to regulate proanthocyanidins accumulation. In particular, yeast two-hybrid assays revealed that ARF2 physically interacts with TT2, a master regulator of proanthocyanidins biosynthesis. Combined together, these results indicated that ARF2 functions as a positive regulator for the fine-tuned spatial and temporal regulation of flavonoids (mainly flavonols and proanthocyanidins) accumulation in Arabidopsis.
学科主题	Plant Sciences
电子版国际标准刊号	1432-2048
出版地	NEW YORK
WOS关键词	RESPONSE FACTOR-2 ARF2 ; SEED COAT DEVELOPMENT ; TRANSPARENT TESTA ; AUXIN TRANSPORT ; TRANSCRIPTION FACTORS ; ANTHOCYANIN BIOSYNTHESIS ; DOMAIN PROTEIN ; COLORFUL MODEL ; MYB DOMAIN ; ACCUMULATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000829614500001
资助机构	National Nature Science Foundation of China [31370335, 31870281] ; Major State Basic Research and Development Program [2013CB127002]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28855]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Wenbo,Xia, Yaying,Su, Xiaojia,et al. ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana[J]. PLANTA,2022,256(2).
APA	Jiang, Wenbo,Xia, Yaying,Su, Xiaojia,&Pang, Yongzhen.(2022).ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana.PLANTA,256(2).
MLA	Jiang, Wenbo,et al."ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana".PLANTA 256.2(2022).