Genome-Wide Analysis of BpYABs and Function Identification Involving in the Leaf and Silique Development in Transgenic Arabidopsis

doi:10.3390/ijms23031670

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	Genome-Wide Analysis of BpYABs and Function Identification Involving in the Leaf and Silique Development in Transgenic Arabidopsis
	Tang, Feng 2; Zhang, Dan ; Chen, Naizhi ; Peng, Xianjun ; Shen, Shihua
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2022
卷号	23 期号:3
关键词	paper mulberry adaxial-abaxial polarity lateral organ development pods and seeds development
DOI	10.3390/ijms23031670
文献子类	Article
英文摘要	YABs play an important role in the leaf development of the paper mulberry (Broussonetia papyrifera) and of the heterophylly. Thus, we investigated the function of BpYABs. Gene cloning, phylogenetic analysis, motif identification, subcellular localization, transactivation activity assay, qRT-PCR, in situ hybridization, and ectopic expression were used in our study. Six BpYABs were isolated, and four of them had transcriptional activity. BpYAB1, BpYAB3, BpYAB4, and BpYAB5 were localized to the nucleus. BpYAB1 was only expressed in the flower, while BpYAB6 was not expressed in any detected tissues; the four remaining BpYABs were expressed in the bud, leaf and flower, and their expression level decreased with leaf development. Further in situ hybridization showed that BpYAB3 and BpYAB5 were expressed in the vascular tissues and lamina, but neither showed the adaxial-abaxial polarity distribution pattern in the mature leaf lamina. Ectopic expression of BpYAB2, BpYAB3, BpYAB4 and BpYAB5 induced increased expression of AtWOX1 and caused the leaf of Arabidopsis to become smaller and curl downwards. Ectopic expression also led to shorter siliques and smaller seeds, but not for BpYAB5. These results suggest that BpYABs have functional divergency and redundancy in regulating leaf and silique development.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
电子版国际标准刊号	1422-0067
出版地	BASEL
WOS关键词	YABBY GENE FAMILY ; FILAMENTOUS-FLOWER ; ECTOPIC EXPRESSION ; ZINC-FINGER ; EVOLUTION ; MERISTEM ; ENCODES ; PROTEIN ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	MDPI
WOS记录号	WOS:000930530800001
资助机构	National Natural Science Foundation of China [31770360, 32070358, 31870247] ; Beijing Natural Science Foundation [6202026]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28762]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Tang, Feng,Zhang, Dan,Chen, Naizhi,et al. Genome-Wide Analysis of BpYABs and Function Identification Involving in the Leaf and Silique Development in Transgenic Arabidopsis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(3).
APA	Tang, Feng,Zhang, Dan,Chen, Naizhi,Peng, Xianjun,&Shen, Shihua.(2022).Genome-Wide Analysis of BpYABs and Function Identification Involving in the Leaf and Silique Development in Transgenic Arabidopsis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(3).
MLA	Tang, Feng,et al."Genome-Wide Analysis of BpYABs and Function Identification Involving in the Leaf and Silique Development in Transgenic Arabidopsis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.3(2022).