Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge

doi:10.3390/antiox11112094

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	Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge
	Zhang, Lianhua 2; Wang, Jian ; Piao, Xiangshu
刊名	ANTIOXIDANTS
	2022
卷号	11 期号:11
关键词	25-hydroxycholecalciferol blood antioxidant status intestinal health microbiota broilers
DOI	10.3390/antiox11112094
文献子类	Article
英文摘要	Our experiment was to detect the effects of 25-hydroxycholecalciferol (25OHD(3)) on antioxidant capacity, immune status and gut health of broilers under lipopolysaccharide (LPS) challenge. In total, 108 male Arbor Acre broilers (48.5 +/- 0.4 g) were allotted to three treatment groups containing six replicates for each group with six birds per replicate: (1) corn-soybean basal diet + injection of sterile saline (CON group); (2) corn-soybean basal diet + an injection of LPS (LPS group); (3) corn-soybean basal diet with 50 mu g/kg 25OHD(3) + injection of LPS (LPS + 25-D group). At the end of the experiment, birds were intraperitoneally injected with LPS in the LPS and LPS + 25-D groups based on the dosage of 5.0 mg/kg BW, or the equivalent volume of 0.9% sterile saline in the CON group. At 4 h postinjection, blood samples, jejunal and ileal tissues and cecal digesta were collected to analyze blood antioxidant capacity, intestinal barrier function and microbiota. The results showed that broilers challenged with LPS had significantly higher BW loss than the CON group, and 25OHD(3) alleviated BW loss induced by the LPS challenge. 25OHD(3) alleviated the LPS-induced decline (p < 0.05) in serum activities of superoxide dismutase (SOD) and immunoglobulin G (IgG), as well as prevented the LPS-induced increase (p < 0.05) in serum content of tumor necrosis factor-alpha (TNF-alpha). 25OHD(3) significantly increased villus height in the jejunum and the relative mRNA abundance of Occludin in the jejunum and ileum, as well as prevented the LPS-induced increase in the jejunal content of interferon-gamma (IFN-gamma) compared with the LPS group. Compared with the LPS group, 25OHD3 significantly increased Lactobacillus abundance and decreased Lachnoclostridium abundance in the cecal digesta, as well as had the potential to enhance metabolite contents including propionate, isobutyrate, butyrate and total SCFA. The correlation analysis revealed that BW loss and serum contents of TNF-alpha, IL-1 beta and D-lactate were positively correlated with Lachnoclostridium and negatively correlated with Lactobacillus (p < 0.05). Overall, 25OHD(3) partially improves the antioxidant status, immunity, intestinal barrier and microbial composition of broilers under the LPS challenge.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
电子版国际标准刊号	2076-3921
出版地	BASEL
WOS关键词	VITAMIN-D ; INFLAMMATORY RESPONSES ; 1,25-DIHYDROXYVITAMIN D-3 ; NUTRIENT DIGESTIBILITY ; PHYSIOLOGICAL STRESS ; OXIDATIVE STRESS ; SUPPLEMENTATION ; CHICKENS ; RECEPTOR ; PIGLETS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	MDPI
WOS记录号	WOS:000880746100001
资助机构	National Natural Science Foundation of China [31772612] ; Beijing Municipal Natural Science Foundation [6202019]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28709]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 2.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lianhua,Wang, Jian,Piao, Xiangshu. Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge[J]. ANTIOXIDANTS,2022,11(11).
APA	Zhang, Lianhua,Wang, Jian,&Piao, Xiangshu.(2022).Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge.ANTIOXIDANTS,11(11).
MLA	Zhang, Lianhua,et al."Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge".ANTIOXIDANTS 11.11(2022).