AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana

doi:10.1093/nar/gkac501

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	AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana
	Xiong, Hai-Bo ; Pan, Hui-Min ; Long, Qiao-Ying ; Wang, Zi-Yuan ; Qu, Wan-Tong ; Mei, Tong ; Zhang, Nan ; Xu, Xiao-Feng ; Yang, Zhong-Nan ; Yu, Qing-Bo
刊名	NUCLEIC ACIDS RESEARCH
	2022
卷号	50 期号:12 页码:6715-6734
ISSN号	0305-1048
DOI	10.1093/nar/gkac501
文献子类	Article
英文摘要	In Escherichia coli, transcription-translation coupling is mediated by NusG. Although chloroplasts are descendants of endosymbiotic prokaryotes, the mechanism underlying this coupling in chloroplasts remains unclear. Here, we report transcription-translation coupling through AtNusG in chloroplasts. AtNusG is localized in chloroplast nucleoids and is closely associated with the chloroplast PEP complex by interacting with its essential component PAP9. It also comigrates with chloroplast ribosomes and interacts with their two components PRPS5 (uS5c) and PRPS10 (uS10c). These data suggest that the transcription and translation machineries are coupled in chloroplasts. In the atnusg mutant, the accumulation of chloroplast-encoded photosynthetic gene transcripts, such as psbA, psbB, psbC and psbD, was not obviously changed, but that of their proteins was clearly decreased. Chloroplast polysomic analysis indicated that the decrease in these proteins was due to the reduced efficiency of their translation in this mutant, leading to reduced photosynthetic efficiency and enhanced sensitivity to cold stress. These data indicate that AtNusG-mediated coupling between transcription and translation in chloroplasts ensures the rapid establishment of photosynthetic capacity for plant growth and the response to environmental changes. Therefore, our study reveals a conserved mechanism of transcription-translation coupling between chloroplasts and E. coli, which perhaps represents a regulatory mechanism of chloroplast gene expression. This study provides insights into the underlying mechanisms of chloroplast gene expression in higher plants.
学科主题	Biochemistry & Molecular Biology
电子版国际标准刊号	1362-4962
出版地	OXFORD
WOS关键词	ACTIVE CHROMOSOME COMPLEX ; RIBOSOMAL-PROTEIN ; FACTOR-NUSG ; INTERACTS ; STRESS ; VISUALIZATION ; COMPONENT ; PLASTIDS ; SEQUENCE ; BINDING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000814749800001
资助机构	National Natural Scientific Foundation of China [31570232, 31930009] ; Shanghai Key Laboratory of Plant Molecular Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28647]
专题	中科院光生物学重点实验室
作者单位	1.Zhang, Nan 2.[Xiong, Hai-Bo 3.Pan, Hui-Min 4.Long, Qiao-Ying 5.Wang, Zi-Yuan 6.Qu, Wan-Tong 7.Mei, Tong 8.Xu, Xiao-Feng 9.Yang, Zhong-Nan 10.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Hai-Bo,Pan, Hui-Min,Long, Qiao-Ying,et al. AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana[J]. NUCLEIC ACIDS RESEARCH,2022,50(12):6715-6734.
APA	Xiong, Hai-Bo.,Pan, Hui-Min.,Long, Qiao-Ying.,Wang, Zi-Yuan.,Qu, Wan-Tong.,...&Yu, Qing-Bo.(2022).AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana.NUCLEIC ACIDS RESEARCH,50(12),6715-6734.
MLA	Xiong, Hai-Bo,et al."AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana".NUCLEIC ACIDS RESEARCH 50.12(2022):6715-6734.