Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1

doi:10.1002/2211-5463.13453

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	Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1
	Wang, Jia ; Li, Xiaoyi 2; Chang, Jing-Wen ; Ye, Tong ; Mao, Ying ; Wang, Xiao ; Liu, Lin
刊名	FEBS OPEN BIO
	2022
卷号	12 期号:9 页码:1677-1687
关键词	Arabidopsis thaliana biliverdin crystallography heme heme oxygenase
ISSN号	2211-5463
DOI	10.1002/2211-5463.13453
文献子类	Article
英文摘要	Arabidopsis thaliana heme oxygenase-1 (AtHO-1), a metabolic enzyme in the heme degradation pathway, serves as a prototype for study of the bilin-related functions in plants. Past biological analyses revealed that AtHO-1 requires ferredoxin-NADP(+) reductase (FNR) and ferredoxin for its enzymatic activity. Here, we characterized the binding and degradation of heme by AtHO-1, and found that ferredoxin is a dispensable component of the reducing system that provides electrons for heme oxidation. Furthermore, we reported the crystal structure of heme-bound AtHO-1, which demonstrates both conserved and previously undescribed features of plant heme oxygenases. Finally, the electron transfer pathway from FNR to AtHO-1 is suggested based on the known structural information.
学科主题	Biochemistry & Molecular Biology
出版地	HOBOKEN
WOS关键词	PHYTOCHROME-CHROMOPHORE BIOSYNTHESIS ; SYNECHOCYSTIS SP PCC-6803 ; BOUND CRYSTAL-STRUCTURES ; RAT HEME ; CORYNEBACTERIUM-DIPHTHERIAE ; CATALYTIC MECHANISM ; ELECTRON-TRANSFER ; PLANT HEME ; COMPLEX ; MUTANT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000813393200001
资助机构	National Natural Science Foundation of China [31800251, 31870729] ; National Key R&D Program of China [2017YFA0503703] ; Anhui Provincial Natural Science Foundation [1908085QC129] ; Undergraduate Innovation and Entrepreneurship Training Program [X202110357056, 202110357051]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28517]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jia,Li, Xiaoyi,Chang, Jing-Wen,et al. Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1[J]. FEBS OPEN BIO,2022,12(9):1677-1687.
APA	Wang, Jia.,Li, Xiaoyi.,Chang, Jing-Wen.,Ye, Tong.,Mao, Ying.,...&Liu, Lin.(2022).Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1.FEBS OPEN BIO,12(9),1677-1687.
MLA	Wang, Jia,et al."Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1".FEBS OPEN BIO 12.9(2022):1677-1687.