Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis

doi:10.1021/acs.jafc.2c06230

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	Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis
	Wu, Shaowen ; Zhang, Qunjie 2; Zhang, Wenyang ; Huang, Wenjie ; Kong, Qian ; Liu, Qinjian ; Li, Wenyan ; Zou, Xinlu ; Liu, Chun-Ming ; Yan, Shijuan
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2022
关键词	Aspergillus flavus aflatoxins imizoquin multiomics thermal proteome profiling
ISSN号	0021-8561
DOI	10.1021/acs.jafc.2c06230
文献子类	Article; Early Access
英文摘要	Oxylipins play important signaling roles in aflatoxin (AF) biosynthesis in Aspergillus flavus. We previously showed that exogenous supply of autoxidated linolenic acid (AL) inhibited AF biosynthesis in A. flavus via oxylipins, but the molecular mechanism is still unknown. Here, we performed multiomics analyses of A. flavus grown in media with or without AL. Targeted metabolite analyses and quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) showed that the imizoquin (IMQ) biosynthetic pathway was distinctly upregulated in the presence of AL. C-13-glucose labeling confirmed in parallel that the tricarboxylic acid cycle was also enhanced by AL, consistent with observed increases in mycelial growth. Moreover, we integrated thermal proteome profiling and molecular dynamics simulations to identify a potential receptor of AL; AL was found to interact with a transporter (ImqJ) located in the IMQ gene cluster, primarily through hydrophobic interactions. Further analyses of strains with an IMQ pathway transcription factor overexpressed or knocked out confirmed that this pathway was critical for AL-mediated inhibition of AF biosynthesis. Comparison of 22 assembled A. flavus and Aspergillus oryzae genomes showed that genes involved in the IMQ pathway were positively selected in A. oryzae. Taken together, the results of our study provide novel insights into oxylipin-mediated regulation of AF biosynthesis and suggest potential methods for preventing AF contamination of crops.
学科主题	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
电子版国际标准刊号	1520-5118
出版地	WASHINGTON
WOS关键词	SIGNALING PATHWAYS ; SOFTWARE NEWS ; FATTY-ACIDS ; DYNAMICS ; PROTEIN ; GENE ; IDENTIFICATION ; METABOLITES ; ANTAGONISM ; MODELS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000895983800001
资助机构	Natural Science Foundation of China ; Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science ; Natural Science Foundation of Guangdong Province ; Guangdong Special Support 318 Program ; [31500045] ; [R2021YJ-YB1004] ; [R2020PY-JX019] ; [2014A030310489] ; [2021TQ06N115]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29030]
专题	中科院植物分子生理学重点实验室
作者单位	1.South China Agr Univ, Inst Genom & Bioinformat, Guangzhou 510642, Peoples R China 2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shaowen,Zhang, Qunjie,Zhang, Wenyang,et al. Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022.
APA	Wu, Shaowen.,Zhang, Qunjie.,Zhang, Wenyang.,Huang, Wenjie.,Kong, Qian.,...&Yan, Shijuan.(2022).Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Wu, Shaowen,et al."Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022).