ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana

doi:10.1186/s12915-022-01455-4

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	ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana
	Chen, Xia 2; Xue, Huidan 3; Zhu, Liping 4; Wang, Huiqin 4; Long, Hao 2; Zhao, Jun 2; Meng, Funing 2; Liu, Yunfei 2; Ye, Yuan 2; Luo, Xiaomin
刊名	BMC BIOLOGY
	2022
卷号	20 期号:1
关键词	ERF49 Heat stress Brassinosteroid (BR) Brassinazole resistant 1 (BZR1) Arabidopsis thaliana
DOI	10.1186/s12915-022-01455-4
文献子类	Article
英文摘要	Background Heat stress is a major abiotic stress affecting the growth and development of plants, including crop species. Plants have evolved various adaptive strategies to help them survive heat stress, including maintaining membrane stability, encoding heat shock proteins (HSPs) and ROS-scavenging enzymes, and inducing molecular chaperone signaling. Brassinosteroids (BRs) are phytohormones that regulate various aspects of plant development, which have been implicated also in plant responses to heat stress, and resistance to heat in Arabidopsis thaliana is enhanced by adding exogenous BR. Brassinazole resistant 1 (BZR1), a transcription factor and positive regulator of BR signal, controls plant growth and development by directly regulating downstream target genes. However, the molecular mechanism at the basis of BR-mediated heat stress response is poorly understood. Here, we report the identification of a new factor critical for BR-regulated heat stress tolerance. Results We identified ERF49 in a genetic screen for proteins required for BR-regulated gene expression. We found that ERF49 is the direct target gene of BZR1 and that overexpressing ERF49 enhanced sensitivity of transgenic plants to heat stress. The transcription levels of heat shock factor HSFA2, heat stress-inducible gene DREB2A, and three heat shock protein (HSP) were significantly reduced under heat stress in ERF49-overexpressed transgenic plants. Transcriptional activity analysis in protoplast revealed that BZR1 inhibits ERF49 expression by binding to the promoter of ERF49. Our genetic analysis showed that dominant gain-of-function brassinazole resistant 1-1D mutant (bzr1-1D) exhibited lower sensitivity to heat stress compared with wild-type. Expressing ERF49-SRDX (a dominant repressor reporter of ERF49) in bzr1-1D significantly decreased the sensitivity of ERF49-SRDX/bzr1-1D transgenic plants to heat stress compared to bzr1-1D. Conclusions Our data provide clear evidence that BR increases thermotolerance of plants by repressing the expression of ERF49 through BZR1, and this process is dependent on the expression of downstream heat stress-inducible genes. Taken together, our work reveals a novel molecular mechanism mediating plant response to high temperature stress.
学科主题	Biology
电子版国际标准刊号	1741-7007
出版地	LONDON
WOS关键词	RESPONSIVE GENE-EXPRESSION ; TRANSCRIPTION FACTOR HSFA3 ; FUNCTIONAL-ANALYSIS ; REPRESSION DOMAIN ; BRASSICA-NAPUS ; GROWTH ; DROUGHT ; CASCADE ; BINDING ; DREB2A
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	BMC
WOS记录号	WOS:000881658500002
资助机构	National Key Research and Development Program [2018YFD100070412, 2020YFA0907600] ; Hainan Yazhou Bay seed laboratory top-ranking project [B21HJ2005] ; National Natural Science Foundation of China [32200444]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28914]
专题	中科院植物分子生理学重点实验室
作者单位	1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 4.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China 5.Shaanxi Normal Univ, Coll Life Sci, Xian 710119, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xia,Xue, Huidan,Zhu, Liping,et al. ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana[J]. BMC BIOLOGY,2022,20(1).
APA	Chen, Xia.,Xue, Huidan.,Zhu, Liping.,Wang, Huiqin.,Long, Hao.,...&Zhu, Shengwei.(2022).ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana.BMC BIOLOGY,20(1).
MLA	Chen, Xia,et al."ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana".BMC BIOLOGY 20.1(2022).