MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple

doi:10.1016/j.plaphy.2022.06.026

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	MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple
	Dong, Haiqiang ; Zheng, Qingbo 2; Zhou, Yufei ; Zhou, Yuwen ; Bao, Zeyang ; Lan, Qingqing ; Li, Xu 1
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2022
卷号	186 页码:11-18
关键词	Apple Adventitious shoot Regeneration MdWOX4-2 MdLBD41
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2022.06.026
文献子类	Article
英文摘要	Apple (Malus domestica Borkh.) is not only an important fruit crop distributed worldwide, but also a common model plant. However, the lack of efficient genetic transformation procedures for apples limits the in-depth studies of their gene functions. Although leaf-regenerated adventitious shoots (LRAS) are a prerequisite for successful genetic transformation of apple, little is known about the underlying molecular mechanism of LRAS. Here, we identified the WUSCHEL-related homeobox (WOX) transcription factor in apple, MdWOX4-2, which was a transcriptional activator. Gene expression as well as morphological and histological observations revealed that MdWOX4-2 is involved in the development of LRAS. Overexpression of MdWOX4-2 conferred higher regenerative capacity in transgenic tobacco (Nicotiana tabacum) as compared to the wild type (WT). The com-bined results of the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), dual luciferase assays, and transient transactivation assay, revealed that MdWOX4-2 directly bound to and activated the MdLBD41 promoter. Moreover, transgenic experiments further demonstrated that MdLBD41 could significantly enhance the formation of adventitious shoot in transgenic tobacco. Collectively, our findings demonstrate that MdWOX4-2 is important for regulating the LRAS development by activating MdLBD41.
学科主题	Plant Sciences
电子版国际标准刊号	1873-2690
出版地	ISSY-LES-MOULINEAUX
WOS关键词	LATERAL ROOT-FORMATION ; WUSCHEL-RELATED HOMEOBOX4 ; CELL-PROLIFERATION ; GENE ; REGENERATION ; LEAF ; WOX4 ; DOWNSTREAM ; ACTIVATION ; REPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000830070200002
资助机构	Zhejiang Provincial Top Discipline of Biological Engineering [(Level A) (ZS2021003), KF2022008] ; China Postdoctoral Science Foundation [2022M713004]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28466]
专题	中科院植物分子生理学重点实验室
作者单位	1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Zhejiang, Peoples R China 2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 10093, Peoples R China
推荐引用方式 GB/T 7714	Dong, Haiqiang,Zheng, Qingbo,Zhou, Yufei,et al. MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,186:11-18.
APA	Dong, Haiqiang.,Zheng, Qingbo.,Zhou, Yufei.,Zhou, Yuwen.,Bao, Zeyang.,...&Li, Xu.(2022).MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple.PLANT PHYSIOLOGY AND BIOCHEMISTRY,186,11-18.
MLA	Dong, Haiqiang,et al."MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple".PLANT PHYSIOLOGY AND BIOCHEMISTRY 186(2022):11-18.