Soil Acidification Under Long-Term N Addition Decreases the Diversity of Soil Bacteria and Fungi and Changes Their Community Composition in a Semiarid Grassland

doi:10.1007/s00248-021-01954-x

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	Soil Acidification Under Long-Term N Addition Decreases the Diversity of Soil Bacteria and Fungi and Changes Their Community Composition in a Semiarid Grassland
	Song, Bing ; Li, Yong 1; Yang, Liuyi 3; Shi, Huiqiu 3; Li, Linghao 3; Bai, Wenming 3; Zhao, Ying
刊名	MICROBIAL ECOLOGY
	2023
卷号	85 期号:1 页码:221-231
关键词	Nitrogen deposition Soil microorganism Bacterial diversity Fungal diversity Community composition Temperate steppe
ISSN号	0095-3628
DOI	10.1007/s00248-021-01954-x
文献子类	Article
英文摘要	Soil microorganisms play key roles in terrestrial biogeochemical cycles and ecosystem functions. However, few studies address how long-term nitrogen (N) addition gradients impact soil bacterial and fungal diversity and community composition simultaneously. Here, we investigated soil bacterial and fungal diversity and community composition based on a long-term (17 years) N addition gradient experiment (six levels: 0, 2, 4, 8, 16, 32 gN m(-2) year(-1)) in temperate grassland, using the high-throughput Illumina MiSeq sequencing. Results showed that both soil bacterial and fungal alpha diversity responded nonlinearly to the N input gradient and reduced drastically when the N addition rate reached 32 g N m(-2) year(-1). The relative abundance of soil bacterial phyla Proteobacteria increased and Acidobacteria decreased significantly with increasing N level. In addition, the relative abundance of bacterial functional groups associated with aerobic ammonia oxidation, aerobic nitrite oxidation, nitrification, respiration of sulfate and sulfur compounds, and chitinolysis significantly decreased under the highest N addition treatment. For soil fungi, the relative abundance of Ascomycota increased linearly along the N enrichment gradient. These results suggest that changes in soil microbial community composition under elevated N do not always support the copiotrophic-oligotrophic hypothesis, and some certain functional bacteria would not simply be controlled by soil nutrients. Further analysis illustrated that reduced soil pH under N addition was the main factor driving variations in soil microbial diversity and community structure in this grassland. Our findings highlight the consistently nonlinear responses of soil bacterial and fungal diversity to increasing N input and the significant effects of soil acidification on soil microbial communities, which can be helpful for the prediction of underground ecosystem processes in light of future rising N deposition.
学科主题	Ecology ; Marine & Freshwater Biology ; Microbiology
电子版国际标准刊号	1432-184X
出版地	NEW YORK
WOS关键词	NITROGEN-FERTILIZATION ; MICROBIAL COMMUNITIES ; DIFFERENTIAL RESPONSES ; BIOMASS ; CARBON ; PLANT ; PH ; AVAILABILITY ; METAANALYSIS ; ENRICHMENT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000743846200002
资助机构	National Natural Science Foundation of China [31800403, 41977009, 41977039] ; Natural Science Foundation of Shandong Province [ZR2020QD115]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29089]
专题	植被与环境变化国家重点实验室
作者单位	1.Ludong Univ, Sch Resources & Environm Engn, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 3.Chinese Acad Forestry, Inst Wetland Res, Beijing Key Lab Wetland Serv & Restorat, Beijing 100091, Peoples R China
推荐引用方式 GB/T 7714	Song, Bing,Li, Yong,Yang, Liuyi,et al. Soil Acidification Under Long-Term N Addition Decreases the Diversity of Soil Bacteria and Fungi and Changes Their Community Composition in a Semiarid Grassland[J]. MICROBIAL ECOLOGY,2023,85(1):221-231.
APA	Song, Bing.,Li, Yong.,Yang, Liuyi.,Shi, Huiqiu.,Li, Linghao.,...&Zhao, Ying.(2023).Soil Acidification Under Long-Term N Addition Decreases the Diversity of Soil Bacteria and Fungi and Changes Their Community Composition in a Semiarid Grassland.MICROBIAL ECOLOGY,85(1),221-231.
MLA	Song, Bing,et al."Soil Acidification Under Long-Term N Addition Decreases the Diversity of Soil Bacteria and Fungi and Changes Their Community Composition in a Semiarid Grassland".MICROBIAL ECOLOGY 85.1(2023):221-231.