Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle | |
Zhou, Yanan1,2,3,4; Zeng, Yan2,3,4; Wang, Peng2,3; Qi, Peng2,3,4; Sun, Yan2,3,4; Zhang, Dun2,3,4 | |
刊名 | SENSORS AND ACTUATORS B-CHEMICAL |
2023-09-01 | |
卷号 | 390页码:8 |
关键词 | Rigid DNA triangle Hemin G-quadruplex Sulfate -reducing bacteria |
DOI | 10.1016/j.snb.2023.133854 |
通讯作者 | Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zengyan@qdio.ac.cn) |
英文摘要 | Here a colorimetric sensing platform for sulfate-reducing bacterial DNA analysis was described by using a catalytic hemin/G-quadruplex loaded rigid DNA triangle assembly. This two-dimensional rigid DNA triangle structure was assembled by target bacterial DNA and three hairpin beacon probes. As three tips of hairpin probes carry specific recognition sites for G-quadruplex formation, target bacterial DNA trigger the opening of hairpin probes, not only forming a steady rigid DNA triangle but also activating the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme. The resultant hemin/G-quadruplex loaded rigid DNA triangle assembly catalyzes a colorimetric reaction with the signal intensity correlated to target concentrations, achieving target bacterial DNA detection with a detection limit as low as 23.99 fM. We further analyzed actual bacteria with this system, by acquiring bacterial DNA including bacteria-specific sequences with polymerase chain reaction (PCR), with the detection limit down to 1 cfu/mL. The system shows good selectivity for the target and resistance to interference, highly stable performance for serum samples application, showing great potential for application in the fields of genetic analysis, environmental monitoring, and medical testing. |
资助项目 | National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] |
WOS关键词 | LABEL-FREE |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE SA |
WOS记录号 | WOS:001010022200001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/182308] |
专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
通讯作者 | Zeng, Yan; Zhang, Dun |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Zhou, Yanan,Zeng, Yan,Wang, Peng,et al. Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,390:8. |
APA | Zhou, Yanan,Zeng, Yan,Wang, Peng,Qi, Peng,Sun, Yan,&Zhang, Dun.(2023).Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle.SENSORS AND ACTUATORS B-CHEMICAL,390,8. |
MLA | Zhou, Yanan,et al."Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle".SENSORS AND ACTUATORS B-CHEMICAL 390(2023):8. |
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