Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?

doi:10.1016/j.jiec.2023.05.008

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	Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?
	Shen, Yuanyuan 1,2,3,4; Sun, Yihan 1,2,3; Wang, Peng 1,2,3; Zhang, Dun 1,2,3
刊名	JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
	2023-08-25
卷号	124 页码:532-538
关键词	Slippery liquid-infused porous surface (SLIPS) Initial adhesion Static condition RNA sequencing (RNA-seq) Type II secretion system (T2SS)
ISSN号	1226-086X
DOI	10.1016/j.jiec.2023.05.008
通讯作者	Wang, Peng()
英文摘要	Slippery liquid-infused porous surfaces (SLIPSs) have distinguished themselves in inhibiting bacteria attachment and biofilm development in static conditions. However, underlying antifouling mechanisms, especially from gene level in bioinformatics, is still lacking. In this work, we investigated the initial attachment difference of Pseudomonas aeruginosa PAO1 on polydimethylsiloxane (PDMS) surface and the infused silicone slippery surface (i-PDMS). RNA sequencing (RNA-seq) was used to investigate the differences in the expression of PAO1 gene on elastomer surface during initial adhesion before and after oil injection. Compared with PDMS, bacterial attachment on i-PDMS was remarkably decreased 98.0 +/- 0.7 % within 10 mins. And the antifouling ability of i-PDMS significantly outperformed PDMS throughout the entire culture period of PAO1 (14 days) in static conditions. RNA-seq reveals that the down-regulated PA1382 of PAO1 in bulk near the i-PDMS surfaces may inhibit bacterial initial adhesion. PA1382 gene encodes type II secretion outer membranes (OM) secretin, also known as type II secretion system (T2SS) protein GspD, which is involved in regulating the opening or closing of exoprotein channels, influencing bacterial adhesion and biofilm formation by controlling the secretion of toxins or effectors. Our findings provide a deeper understanding of the mechanism by which SLIPS inhibits initial bacterial adhesion. (c) 2023 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[U22A20112] ; National Natural Science Foundation of China[52205227] ; Shandong Pro- vince Natural Science Foundation[ZR2020KE008] ; China Postdoctoral Science Foundation[2022M713181] ; Postdoctoral Innovative Talents Support Program of Shandong Province[SDBX2021006] ; Nantong Scien- tific Plan Foundation[JC12022099]
WOS关键词	II SECRETION ; PSEUDOMONAS-AERUGINOSA ; SURFACES ; BACTERIA ; SYSTEM ; PERFORMANCE ; MECHANISM ; ROUGHNESS ; SILICONE ; BIOFILMS
WOS研究方向	Chemistry ; Engineering
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:001008795500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/182270]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Wang, Peng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 168 Wenhai Middle Rd, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Shen, Yuanyuan,Sun, Yihan,Wang, Peng,et al. Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?[J]. JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY,2023,124:532-538.
APA	Shen, Yuanyuan,Sun, Yihan,Wang, Peng,&Zhang, Dun.(2023).Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?.JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY,124,532-538.
MLA	Shen, Yuanyuan,et al."Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?".JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY 124(2023):532-538.