The ancient CgPEPCK-1, not CgPECK-2, evolved into a multifunctional molecule as an intracellular enzyme and extracellular PRR

doi:10.1016/j.dci.2023.104722

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	The ancient CgPEPCK-1, not CgPECK-2, evolved into a multifunctional molecule as an intracellular enzyme and extracellular PRR
	Yin, Xiaoting 2,3,4; Qiu, Limei 2,3; Long, Dandan 2,3; Lv, Zhao 2,3; Liu, Qing 2,3; Wang, Senyu 1,2; Zhang, Weiqian 2; Zhang, Kexin 1,2; Xie, Mengxi 2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2023-08-01
卷号	145 页码:13
关键词	PEPCK Gene evolution and differentiation PRR Catalyzing enzyme
ISSN号	0145-305X
DOI	10.1016/j.dci.2023.104722
通讯作者	Qiu, Limei(qiulimei@qdio.ac.cn)
英文摘要	Phosphoenolpyruvate carboxykinase (PEPCK) is a well-known lyase involved in gluconeogenesis, while their evolution and function differentiation have not been fully understood. In this study, by constructing a phylogenetic tree to examine PEPCKs throughout the evolution from poriferans to vertebrates, Mollusk was highlighted as the only phylum to exhibit two distinct lineages, Mollusca_PEPCK-1 and Mollusca_PEPCK-2. Further study of two representative members from Crassostrea gigas (CgPEPCK-1 and CgPEPCK-2) showed that they both shared conserved sequences and structural characteristics of the catalytic enzyme, while CgPEPCK-2 displayed a higher expression level than CgPEPCK-1 in all tested tissues, and CgPEPCK-1 was specifically implicated in the immune defense against LPS stimulation and Vibrio splendidus infection. Functional analysis revealed that CgPEPCK-2 had stronger enzymatic activity than CgPEPCK-1, while CgPEPCK-1 exhibited stronger binding activity with various PAMPs, and only the protein of CgPEPCK-1 increased significantly in hemolymph during immune stimulation. All results supported that distinct sequence and function differentiations of the PEPCK gene family should have occurred since Mollusk. The more advanced evolutionary branch Mollusca_PEPCK-2 should preserve its essential function as a catalytic enzyme to be more specialized and efficient, while the ancient branch Mollusca_PEPCK-1 probably contained some members, such as CgPEPCK-1, that should be integrated into the immune system as an extracellular immune recognition receptor.
资助项目	National Natural Science Foundation of China[32072999]
WOS关键词	C-TYPE LECTIN ; PHOSPHOENOLPYRUVATE CARBOXYKINASE PEPCK ; INFLAMMATORY CASPASES ; VIBRIO-SPLENDIDUS ; STRESS-RESPONSE ; PROTEIN ; GTP ; MITOCHONDRIAL ; EXPRESSION ; INTEGRIN
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:001000706600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/183204]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Qiu, Limei
作者单位	1.Qingdao Agr Univ, Sch Marine Biol & Engn, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yin, Xiaoting,Qiu, Limei,Long, Dandan,et al. The ancient CgPEPCK-1, not CgPECK-2, evolved into a multifunctional molecule as an intracellular enzyme and extracellular PRR[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023,145:13.
APA	Yin, Xiaoting.,Qiu, Limei.,Long, Dandan.,Lv, Zhao.,Liu, Qing.,...&Xie, Mengxi.(2023).The ancient CgPEPCK-1, not CgPECK-2, evolved into a multifunctional molecule as an intracellular enzyme and extracellular PRR.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,145,13.
MLA	Yin, Xiaoting,et al."The ancient CgPEPCK-1, not CgPECK-2, evolved into a multifunctional molecule as an intracellular enzyme and extracellular PRR".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 145(2023):13.