Potential Scalpel: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results

	Potential Scalpel: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results
	Jia, Conghui; Shi, Longhua; Li, Yuechun; Tian, Yanli; Liu, Sijie; Wang, Shaochi; Liao, Xingrui; Wu, Haofen; Wang, Ziqi; Sun, Jing
刊名	ANALYTICAL CHEMISTRY
	2023
英文摘要	It is of great importance to overcome potential incompatibility problems between dyestuffs and antibodies (mAbs) for extensive commercial application of a dyestuff-chemistry-based ultrafast colorimetric lateral flow immunoassay (cLFIA). Herein, inspired by traditional staining technologies, a basic dyestuff gallocyanine (GC)-assisted biogenic potential scalpel-based cLFIA (GC-ABPS-based cLFIA) by employing clenbuterol (CLE) as proof-of-concept was proposed to solve a high degree of incompatibility between the same potential dyestuffs and mAbs. Goat antimouse immunoglobulin (Ab2) could serve as the potential scalpel to form the positive potential value biomolecular network self-assemblers (BNSA) with anti-CLE mAbs (AbCLE) by noncovalent force. The cLFIA completed the entire detection process from de novo to detection results within 30 min thanks to the easy availability and ideal marking efficiency (<= 1 min, saving 0.4-10 h) of GC. Encouragingly, the proposed ultrafast GC-ABPSbased cLFIA has also exhibited high sensitivity (0.411 ng mL-1) and low cost (300 times) compared with other cLFIAs. Also, the feasibility of the proposed cLFIA was demonstrated by detecting CLE in beef, pork ham, and skim milk. Finally, the proposed GCABPS-based cLFIA has broadened the application range of dyestuffs and provided an effective reference strategy for the application of dyestuffs in food safety monitoring.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61694]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jia, Conghui,Shi, Longhua,Li, Yuechun,et al. Potential Scalpel: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results[J]. ANALYTICAL CHEMISTRY,2023.
APA	Jia, Conghui.,Shi, Longhua.,Li, Yuechun.,Tian, Yanli.,Liu, Sijie.,...&Wang, Jianlong.(2023).Potential Scalpel: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results.ANALYTICAL CHEMISTRY.
MLA	Jia, Conghui,et al."Potential Scalpel: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results".ANALYTICAL CHEMISTRY (2023).