Nuclear magnetic resonance-based solvent system selection for counter-current chromatography separation of compounds present in the same high-performance liquid chromatography peak: Flavonoids in barley seedlings as an example

	Nuclear magnetic resonance-based solvent system selection for counter-current chromatography separation of compounds present in the same high-performance liquid chromatography peak: Flavonoids in barley seedlings as an example
	Chen, Tao; Jia, Jing; Zou, Denglang; Shen, Cheng; Wang, Shuo; Gao, Xiuzhen; Yun, Zhongxiang; Ma, Jialin; Zhao, Jing; Wang, Huan
刊名	JOURNAL OF SEPARATION SCIENCE
	2023
英文摘要	Partition coefficient is a key parameter for counter-current chromatography separation. High-performance liquid chromatography (HPLC) is the most commonly used tool for the screening of partition coefficients. However, HPLC technology is not applicable to the compounds present in the same chromatographic peak. Nuclear magnetic resonance (NMR) technology could easily distinguish compounds according to their characteristic absorption even if they exist in the same HPLC peak. In this study, two flavonoids present in the same HPLC peak were successfully purified by counter-current chromatography with a solvent system screened by NMR to show the great potential of NMR technology in the screening of the partition coefficient of co-efflux compounds. Through NMR screening, an optimized ethyl acetate/n-buthanol/water (7:3:10, v/v/v) system was applied in this study. As a result, two flavonoids, including 4.8 mg of 3 & PRIME;-methoxyl-6 & PRIME;''-O-feruloylsaponarin and 9.8 mg of 6 & PRIME;''-O-feruloylsaponarin were separated from 15 mg of the mixture. There is only one methoxy group difference between the two flavonoids. This study provides a new strategy for the screening of counter-current chromatography solvent systems and broadens the application scope of counter-current chromatography.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61605]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Tao,Jia, Jing,Zou, Denglang,et al. Nuclear magnetic resonance-based solvent system selection for counter-current chromatography separation of compounds present in the same high-performance liquid chromatography peak: Flavonoids in barley seedlings as an example[J]. JOURNAL OF SEPARATION SCIENCE,2023.
APA	Chen, Tao.,Jia, Jing.,Zou, Denglang.,Shen, Cheng.,Wang, Shuo.,...&Li, Yulin.(2023).Nuclear magnetic resonance-based solvent system selection for counter-current chromatography separation of compounds present in the same high-performance liquid chromatography peak: Flavonoids in barley seedlings as an example.JOURNAL OF SEPARATION SCIENCE.
MLA	Chen, Tao,et al."Nuclear magnetic resonance-based solvent system selection for counter-current chromatography separation of compounds present in the same high-performance liquid chromatography peak: Flavonoids in barley seedlings as an example".JOURNAL OF SEPARATION SCIENCE (2023).