Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection

doi:10.1016/j.snb.2023.134423

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	Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection
	Zeng, Yan 1,2,3; Qi, Peng 1,2,3; Chen, Jiawei 1,2,3; Wang, Peng 1,2,3; Zhang, Dun 1,2,3
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2023-12-01
卷号	396 页码:7
关键词	DNA hyperbranched nanostructure SERS Target aided Bacterial 16 S rDNA genes
DOI	10.1016/j.snb.2023.134423
通讯作者	Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要	Quantity analysis of bacterial 16 S rDNA gene is still a challenge. Here we successfully achieved bacterial 16 S rDNA gene SERS analysis with target aided self-assembly of DNA hyperbranched nanostructures. Catalytic hairpin assembly (CHA) was first introduced for self-assembly of branched nanostructures, including target bacterial DNA served as trigger probe and three hairpin probes for further target activated cross opening. Rolling cycle amplification (RCA) was then introduced by using a circular DNA as template and single stranded branches of the formed branched nanostructures as primer, forming a concatemer containing multiple copies of tandem repeating segments with same sequences complementary to the circular templates. After that, thousands of Raman-Au nanoprobe would hybridize this long single stranded DNAs for DNA-AuNPs nanoaggretates assembly. By using SERS signal collection, target bacterial DNAs would be successfully analyzed with ultra-sensitivity as low as 15.0 fM. Furthermore, selectivity test for targets with mismatched DNA sequences and other bacterial 16 S rDNA genes show good results. This sensing method was extended to quantify target genes in human plasma samples, showing its excellent applicability in complex samples.
资助项目	National Natural Science Foundation of China[42376208] ; National Natural Science Foundation of China[41922040]
WOS关键词	GOLD NANOPARTICLES ; AMPLIFICATION
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:001075737400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/181706]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Qi, Peng; Zhang, Dun
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Yan,Qi, Peng,Chen, Jiawei,et al. Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,396:7.
APA	Zeng, Yan,Qi, Peng,Chen, Jiawei,Wang, Peng,&Zhang, Dun.(2023).Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection.SENSORS AND ACTUATORS B-CHEMICAL,396,7.
MLA	Zeng, Yan,et al."Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection".SENSORS AND ACTUATORS B-CHEMICAL 396(2023):7.