Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum

doi:10.1016/j.algal.2023.103270

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum
	Yanga, Wenting 1,3,4; Zhou, Lu 2,3,4; Zhuo, Jintao 2,3,4; Li, Xin 2,3,4; Wu, Songcui 3,4; Yu, Guihong ; Gao, Shan 3,4; Wang, Guangce 3,4
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2023-09-01
卷号	75 页码:7
关键词	Phaeodactylum tricornutum Secretory antimicrobial peptide Antibacterial
ISSN号	2211-9264
DOI	10.1016/j.algal.2023.103270
通讯作者	Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Antimicrobial peptides (AMPs) are small-molecule polypeptides that occur widely in nature, have a broad antibacterial spectrum, and do not induce drug resistance in bacteria. Therefore, they are potential substitutes for antibiotics. However, there have been few reports of the production of the AMPs secreted by diatoms. Phaeodactylum tricornutum is a model diatom species for which protocols for its transformation are well established, and it can be directly used as fish and shrimp bait. In this study, we used P. tricornutum as a cell factory to produce secreted AMPs extracellularly. We also constructed an expression vector containing the secretory promoter and signal peptide of the Scygonadin gene, and successfully expressed AMPs in P. tricornutum, confirmed at the levels of DNA, RNA, and protein. Bacteriostatic experiments showed that the secreted AMPs exerted an obvious antibacterial effect on Kocuria sp. and Trelief5 alpha Chemical Competent Cells. This study demonstrates that P. tricornutum effectively expresses and secretes AMPs with obvious antibacterial effects.
资助项目	National Natural Science Founda- tion of China[41976097] ; National Key R & D Program of China[2018YFD0901500]
WOS关键词	TRANSFORMATION ; MECHANISMS ; GENE
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001083333200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/181622]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Gao, Shan; Wang, Guangce
作者单位	1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yanga, Wenting,Zhou, Lu,Zhuo, Jintao,et al. Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,75:7.
APA	Yanga, Wenting.,Zhou, Lu.,Zhuo, Jintao.,Li, Xin.,Wu, Songcui.,...&Wang, Guangce.(2023).Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,75,7.
MLA	Yanga, Wenting,et al."Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 75(2023):7.