Heat sensitive E-helix cut ferritin nanocages for facile and high-efficiency loading of doxorubicin

doi:10.1016/j.ijbiomac.2023.126973

	Heat sensitive E-helix cut ferritin nanocages for facile and high-efficiency loading of doxorubicin
	Xia, Haining 2,3; Xu, Huangtao 3; Wang, Jiarong 3; Wang, Changhao 2,3; Chen, Ruiguo 3; Tao, Tongxiang 2,3; Xu, Shuai 3; Zhang, Jing 2,3; Ma, Kun 3; Wang, Junfeng 1,2,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2023-12-31
卷号	253
关键词	Ferritin nanocages Drug loading Structural modification Thermal response Ion-assisted
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2023.126973
通讯作者	Ma, Kun(makun@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn)
英文摘要	Ferritin possesses a stable and uniform cage structure, along with tumor-targeting properties and excellent biocompatibility, making it a promising drug delivery vehicle. However, the current ferritin drug loading strategy involves complex steps and harsh reaction conditions, resulting in low yield and recovery of drug loading, which limits the clinical application prospects of ferritin nanomedicine. In this study, we utilized the high-efficiency heat-sensitivity of the multiple channel switch structures of the E-helix-cut ferritin mutant (EcutHFn) and Cu2+ assistance to achieve high-efficiency loading of chemotherapeutic drugs in a one-step process at low temperatures. This method features mild reaction conditions (45 degrees C), high loading efficiency (about 110 doxorubicin (Dox) per Ecut-HFn), and improved protein and Dox recovery rates (with protein recovery rate around 94 % and Dox recovery rate reaching up to 45 %). The prepared ferritin-Dox particles (Ecut-HFn-Cu-Dox) exhibit a uniform size distribution, good stability, and retain the natural tumor targeting ability of ferritin. Overall, this temperature-controlled drug loading strategy utilizing heat-sensitivity ferritin mutants is energy saving, environmentally friendly, efficient, and easy to operate, offering a new perspective for scaling up the industrial production of ferritin drug carriers.
资助项目	HFIPS Director's Fund[YZJJQY202201] ; Research Fund of Anhui Institute of Translational Medicine[2021zhyx-C44] ; National Natural Science Foundation of China[3190110313] ; Hefei Municipal Natural Science Foundation[2022049] ; Special Foundation of President of the Chinese Academy of Sciences[YZJJ2022QN44] ; High Magnetic Field Laboratory of Anhui Province
WOS关键词	PHOTODYNAMIC THERAPY ; PROTEIN NANOCAGES ; LEUCINE-ZIPPER ; H-FERRITIN ; TUMOR ; NANOPARTICLES ; DELIVERY ; ENCAPSULATION ; PHYTOFERRITIN ; NANOPROBES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001082943800001
资助机构	HFIPS Director's Fund ; Research Fund of Anhui Institute of Translational Medicine ; National Natural Science Foundation of China ; Hefei Municipal Natural Science Foundation ; Special Foundation of President of the Chinese Academy of Sciences ; High Magnetic Field Laboratory of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/133499]
专题	中国科学院合肥物质科学研究院
通讯作者	Ma, Kun; Wang, Junfeng
作者单位	1.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, CAS Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xia, Haining,Xu, Huangtao,Wang, Jiarong,et al. Heat sensitive E-helix cut ferritin nanocages for facile and high-efficiency loading of doxorubicin[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,253.
APA	Xia, Haining.,Xu, Huangtao.,Wang, Jiarong.,Wang, Changhao.,Chen, Ruiguo.,...&Wang, Junfeng.(2023).Heat sensitive E-helix cut ferritin nanocages for facile and high-efficiency loading of doxorubicin.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,253.
MLA	Xia, Haining,et al."Heat sensitive E-helix cut ferritin nanocages for facile and high-efficiency loading of doxorubicin".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 253(2023).