Complete genome analysis of pathogenic Metschnikowia bicuspidata strain MQ2101 isolated from diseased ridgetail white prawn, Exopalaemon carinicauda

doi:10.1186/s12866-023-02865-2

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Complete genome analysis of pathogenic Metschnikowia bicuspidata strain MQ2101 isolated from diseased ridgetail white prawn, Exopalaemon carinicauda
	Shi, Wen-jun 2,3,4; Zhao, Ran 1,3; Zhu, Jian-qiang 1,3; Wan, Xi-he 3; Wang, Li-bao 3; Li, Hui 3; Qin, Song 2,4
刊名	BMC MICROBIOLOGY
	2023-04-29
卷号	23 期号:1 页码:17
关键词	Exopalaemon carinicauda Metschnikowia bicuspidata Complete genome sequence Virulence genes Metabolic mechanism
ISSN号	1471-2180
DOI	10.1186/s12866-023-02865-2
通讯作者	Wan, Xi-he(wxh1708@163.com) ; Qin, Song(sqin@yic.ac.cn)
英文摘要	BackgroundMetschnikowia bicuspidata is a pathogenic yesst that can cause disease in many different economic aquatic animal species. In recent years, there was a new disease outbreak in ridgetail white prawn (Exopalaemon carinicauda) in coastal areas of Jiangsu Province China that was referred to as zombie disease by local farmers. The pathogen was first isolated and identified as M. bicuspidata. Although the pathogenicity and pathogenesis of this pathogen in other animals have been reported in some previous studies, research on its molecular mechanisms is still very limited. Therefore, a genome-wide study is necessary to better understand the physiological and pathogenic mechanisms of M. bicuspidata.ResultIn this study, we obtained a pathogenic strain, MQ2101, of M. bicuspidata from diseased E. carinicauda and sequenced its whole genome. The size of the whole genome was 15.98 Mb, and it was assembled into 5 scaffolds. The genome contained 3934 coding genes, among which 3899 genes with biological functions were annotated in multiple underlying databases. In KOG database, 2627 genes were annotated, which were categorized into 25 classes including general function prediction only, posttranslational modification, protein turnover, chaperones, and signal transduction mechanisms. In KEGG database, 2493 genes were annotated, which were categorized into five classes, including cellular processes, environmental information processing, genetic information processing, metabolism and organismal systems. In GO database, 2893 genes were annotated, which were mainly classified in cell, cell part, cellular processes and metabolic processes. There were 1055 genes annotated in the PHI database, accounting for 26.81% of the total genome, among which 5 genes were directly related to pathogenicity (identity >= 50%), including hsp90, PacC, and PHO84. There were also some genes related to the activity of the yeast itself that could be targeted by antiyeast drugs. Analysis based on the DFVF database showed that strain MQ2101 contained 235 potential virulence genes. BLAST searches in the CAZy database showed that strain MQ2101 may have a more complex carbohydrate metabolism system than other yeasts of the same family. In addition, two gene clusters and 168 putative secretory proteins were predicted in strain MQ2101, and functional analysis showed that some of the secretory proteins may be directly involved in the pathogenesis of the strain. Gene family analysis with five other yeasts revealed that strain MQ2101 has 245 unique gene families, including 274 genes involved in pathogenicity that could serve as potential targets.ConclusionGenome-wide analysis elucidated the pathogenicity-associated genes of M. bicuspidate while also revealing a complex metabolic mechanism and providing putative targets of action for the development of antiyeast drugs for this pathogen. The obtained whole-genome sequencing data provide an important theoretical basis for transcriptomic, proteomic and metabolic studies of M. bicuspidata and lay a foundation for defining its specific mechanism of host infestation.
WOS关键词	ALDO-KETO REDUCTASE ; DRUG-RESISTANCE ; YEAST ; GENE ; HSP90 ; LYSOPHOSPHOLIPASE ; IDENTIFICATION ; SUPERFAMILY ; DISRUPTION ; METABOLISM
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000978482800001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/32893]
专题	海岸带生物学与生物资源利用重点实验室
通讯作者	Wan, Xi-he; Qin, Song
作者单位	1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Inst Oceanol & Marine Fisheries, 31, Jiaoyu Rd, Nantong 226007, Jiangsu, Peoples R China 4.Yantai Inst Coastal Zone Res, Chinese Acad Sci, Key Lab Biol & Bioresource Utilizat, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Shi, Wen-jun,Zhao, Ran,Zhu, Jian-qiang,et al. Complete genome analysis of pathogenic Metschnikowia bicuspidata strain MQ2101 isolated from diseased ridgetail white prawn, Exopalaemon carinicauda[J]. BMC MICROBIOLOGY,2023,23(1):17.
APA	Shi, Wen-jun.,Zhao, Ran.,Zhu, Jian-qiang.,Wan, Xi-he.,Wang, Li-bao.,...&Qin, Song.(2023).Complete genome analysis of pathogenic Metschnikowia bicuspidata strain MQ2101 isolated from diseased ridgetail white prawn, Exopalaemon carinicauda.BMC MICROBIOLOGY,23(1),17.
MLA	Shi, Wen-jun,et al."Complete genome analysis of pathogenic Metschnikowia bicuspidata strain MQ2101 isolated from diseased ridgetail white prawn, Exopalaemon carinicauda".BMC MICROBIOLOGY 23.1(2023):17.