NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes | |
Zhen Wang3,4; Wen Ding2; Maosen Ruan4; Yong Liu3,4; Jing Yang4; Huiqin Zhang1,4; Bing Shen2; Junfeng Wang1,3,4; Yunyan Li4 | |
刊名 | biomolecules |
2023-04-22 | |
关键词 | Mpc PRE NMR patch-clamp oligomeric state protein interactions |
ISSN号 | 2218-273X |
DOI | doi.org/10.3390/ biom13050719 |
英文摘要 | The mitochondrial pyruvate carrier (Mpc) plays an indispensable role in the transport of pyruvates across the mitochondrial inner membrane. Despite the two distinct homologous proteins, Mpc1 and Mpc2, were identified in 2012, there are still controversies on the basic functional units and oligomeric state of Mpc complexes. In this study, yeast Mpc1 and Mpc2 proteins were expressed in a prokaryotic heterologous system. Both homo- and hetero-dimers were successfully reconstituted in mixed detergents. Interactions among Mpc monomers were recorded utilizing paramagnetic relaxation enhancement (PRE) nuclear magnetic resonance (NMR) methods. By single-channel patchclamp assays, we discovered that both the Mpc1–Mpc2 hetero-dimer and Mpc1 homo-dimer are able to transport K+ ions. Furthermore, the Mpc1–Mpc2 hetero-dimer demonstrated the ability to transport pyruvates, at a rate significantly higher than that of the Mpc1 homo-dimer, indicating that it could be the basic functional unit of Mpc complexes. Our findings provide valuable insights for further structural determination and the study of the transport mechanism of Mpc complexes. |
URL标识 | 查看原文 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/133240] |
专题 | 合肥物质科学研究院_中科院强磁场科学中心 |
通讯作者 | Zhen Wang; Yunyan Li |
作者单位 | 1.Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China 2.School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; shenbing@ahmu.edu.cn 3.Hefei Institutes of Physical Science (Branch of Graduate School), University of Science and Technology of China, Hefei 230026, China 4.High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China |
推荐引用方式 GB/T 7714 | Zhen Wang,Wen Ding,Maosen Ruan,et al. NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes[J]. biomolecules,2023. |
APA | Zhen Wang.,Wen Ding.,Maosen Ruan.,Yong Liu.,Jing Yang.,...&Yunyan Li.(2023).NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes.biomolecules. |
MLA | Zhen Wang,et al."NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes".biomolecules (2023). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论