Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores

doi:10.1016/j.revpalbo.2023.104946

	Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores
	Hu, Zhen-Zhen 2; Jia, Xin-Lei 2; Chen, Xiao 2; Yang, Zhong-nan 2; Mao, Li-Mi 1; Xue, Jing-Shi 2
刊名	REVIEW OF PALAEOBOTANY AND PALYNOLOGY
	2023-09-01
卷号	316 页码:10
ISSN号	0034-6667
DOI	10.1016/j.revpalbo.2023.104946
通讯作者	Mao, Li-Mi(lmmao@nigpas.ac.cn) ; Xue, Jing-Shi(xuejingshi@shnu.edu.cn)
英文摘要	Spores/pollen records abundant information of biodiversity, evolution, and climate condition. The exine of spores/pollen is mainly composed of sporopollenin, which is chemically stable over geological time. Morphological study of the exine is a classical method used in palynology. In this study, we developed a fluorescence lifetime imaging (FLIM) method to investigate the numbers and lifetimes of fluorophores in sporopollenin. We used 20 pollen grains of each species to study sporopollenin FLIM. The sporopollenin was purified using the acetolysis method. Laser scanning confocal microscopy (LSCM) was used to study the autofluorescence of sporopollenin. The sporopollenin FLIM of pollen grains collected in different years/geographical populations showed no significant difference. Three fluorophores were estimated in the sporopollenin of all the tested plants, including angiosperms (Oryza sativa and Zea mays, Brassica napus and Arabidopsis thaliana), gymnosperms (Cryptomeria fortune and Metasequoia glyptostroboides), ferns (Lygodium japonicum and Ceratopteris thalictroides), and lycophyte (Lycopodium clavatum). Quantitative analysis demonstrated that the lifetimes of fluorophores varied in different species, showing that FLIM provides additional information to distinguish spores/pollen. Further analysis showed significant differences in sporopollenin fluorescence lifetimes between morphologically similar pollen grains of cultivated crops and their wild relatives of the genera Oryza and Triticum. These results show that sporopollenin FLIM offers a new dimension of information on spores/pollen. This new technique has a big potential to fill a gap for traditional morphological comparison to identify dispersed fossil pollen and spores.
资助项目	National Natural Science Foundation of China[31900165] ; National Natural Science Foundation of China[41372170] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB26000000] ; State Key Laboratory of Palaeobiology and Stratigraphy[20211103]
WOS关键词	AUTOFLUORESCENCE ; RESOLUTION ; FERNS ; WALL ; CHEMOTAXONOMY ; SPECTROSCOPY ; MICROSCOPY ; MORPHOLOGY ; PHYLOGENY ; EVOLUTION
WOS研究方向	Plant Sciences ; Paleontology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001063721000001
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Palaeobiology and Stratigraphy
内容类型	期刊论文
源URL	[http://ir.nigpas.ac.cn/handle/332004/42721]
专题	中国科学院南京地质古生物研究所
通讯作者	Mao, Li-Mi; Xue, Jing-Shi
作者单位	1.Chinese Acad Sci, State Key Lab Palaeobiol & Stratig, Nanjing Inst Geol & Palaeontol, Nanjing 210008, Peoples R China 2.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Hu, Zhen-Zhen,Jia, Xin-Lei,Chen, Xiao,et al. Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores[J]. REVIEW OF PALAEOBOTANY AND PALYNOLOGY,2023,316:10.
APA	Hu, Zhen-Zhen,Jia, Xin-Lei,Chen, Xiao,Yang, Zhong-nan,Mao, Li-Mi,&Xue, Jing-Shi.(2023).Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores.REVIEW OF PALAEOBOTANY AND PALYNOLOGY,316,10.
MLA	Hu, Zhen-Zhen,et al."Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores".REVIEW OF PALAEOBOTANY AND PALYNOLOGY 316(2023):10.