Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)

doi:10.3390/antiox12061227

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)
	Li, Yanan 4; Chen, Zongfu 4; Zhang, Peng 3; Gao, Feng 4; Wang, Junfeng 2; Lin, Li 4; Zhang, Haibin 1
刊名	ANTIOXIDANTS
	2023-06-01
卷号	12 期号:6 页码:15
关键词	Holothuroidea deep sea enzyme expression purification
DOI	10.3390/antiox12061227
通讯作者	Zhang, Haibin(hzhang@idsse.ac.cn)
英文摘要	At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 & DEG;C, and it maintains high activity in the range of 0-60 degrees C. It also has high thermal stability when incubated at 37 & DEG;C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
资助项目	National Natural Science Foundation of China[42006115] ; Guangdong Province Basic and Applied Basic Research Fund Project[2020A1515110826] ; Major Scientific and Technological Projects of the Hainan Province[ZDKJ2021036] ; Guangdong Key Laboratory of Marine Materia Medica[LMM2021-5] ; Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[FEEL-2021-10] ; Key Projects of Guangdong Province College Student Innovation and Entrepreneurship Training Program[S202211347043]
WOS关键词	MANGANESE-SUPEROXIDE ; OXIDATIVE STRESS ; REACTIVE OXYGEN ; CLONING ; EXPRESSION ; CDNA
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
语种	英语
出版者	MDPI
WOS记录号	WOS:001016834800001
资助机构	National Natural Science Foundation of China ; Guangdong Province Basic and Applied Basic Research Fund Project ; Major Scientific and Technological Projects of the Hainan Province ; Guangdong Key Laboratory of Marine Materia Medica ; Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment ; Key Projects of Guangdong Province College Student Innovation and Entrepreneurship Training Program
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10380]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China 3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China 4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China
推荐引用方式 GB/T 7714	Li, Yanan,Chen, Zongfu,Zhang, Peng,et al. Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)[J]. ANTIOXIDANTS,2023,12(6):15.
APA	Li, Yanan.,Chen, Zongfu.,Zhang, Peng.,Gao, Feng.,Wang, Junfeng.,...&Zhang, Haibin.(2023).Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus).ANTIOXIDANTS,12(6),15.
MLA	Li, Yanan,et al."Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)".ANTIOXIDANTS 12.6(2023):15.