A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm

doi:10.1016/j.talanta.2023.124922

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部

	A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm
	Wang, Chuanliang 2,3; Geng, Xuhui 3; Gao, Yan 3; Zheng, Chao 3; Guan, Yafeng 1,3
刊名	TALANTA
	2023-12-01
卷号	265 页码:6
关键词	96-Well plate UV fluorometer Micro fluorescence detector array Dynamic zero correction algorithm
ISSN号	0039-9140
DOI	10.1016/j.talanta.2023.124922
通讯作者	Geng, Xuhui(xhgeng@dicp.ac.cn) ; Guan, Yafeng(guanyafeng@dicp.ac.cn)
英文摘要	A 96-well plate UV fluorometer was developed and evaluated. Eight micro fluorescence detectors close to each other were used as detector array for 8 channels. Each detector employed an UV light emitting diode (LED) as light source and a photodiode (PD) with an amplifier circuit as optoelectronic detector. The optical paths of the detectors were designed by ray tracing method to avoid crosstalk between wells. Simultaneously scanning and detecting of 8 channels saves scanning time and improves detection efficiency. The scanning time of the 96-well plate was about 80 s. A dynamic zero correction algorithm was proposed to solve the problem of measurement accuracy reduction caused by the background fluorescence differences between plates and wells under irradia-tion of UV light. The measurement repeatability (RSD) for 1 & mu;g/L 7-Diethylamino-4-methylcoumarin sample was 2.25%. Compared with the fixed zero correction method, the limit of detection (LOD), measurement repeat-ability, and average relative error were improved by 3.3, 2.7, and 4.5 times, respectively. The proposed method is robust and can be applied to different analysis systems. The developed fluorometer has great potential in high-throughput rapid detection of food safety and life sciences.
资助项目	General Program of the National Natural Science Foundation of China[22074146] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2019186] ; DICP Innovation Funding[I202014] ; DICP[DICP I202014]
WOS关键词	LIGHT-EMITTING DIODE ; DETECTION SYSTEM ; READER
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001048128700001
资助机构	General Program of the National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; DICP Innovation Funding ; DICP
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10445]
专题	深海科学研究部
通讯作者	Geng, Xuhui; Guan, Yafeng
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, 28 Luhuitou Rd, Sanya 572000, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Deep Sea Composit Detect Technol Liaoning, Dept Instrumentat & Analyt Chem,CAS Key Lab Separa, 457 Zhongshan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chuanliang,Geng, Xuhui,Gao, Yan,et al. A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm[J]. TALANTA,2023,265:6.
APA	Wang, Chuanliang,Geng, Xuhui,Gao, Yan,Zheng, Chao,&Guan, Yafeng.(2023).A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm.TALANTA,265,6.
MLA	Wang, Chuanliang,et al."A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm".TALANTA 265(2023):6.