Molecular imprinted electrochemical sensor for ovalbumin detection based on boronate affinity and signal amplification approach

doi:10.1016/j.foodchem.2022.135292

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular imprinted electrochemical sensor for ovalbumin detection based on boronate affinity and signal amplification approach
	Fu, Hao 3; Bai, Zhenyu 3; Li, Ping 3; Feng, Xiaoyang 3; Hu, Xueping 3; Song, Xingliang 3; Chen, Lingxin 1,2
刊名	FOOD CHEMISTRY
	2023-05-30
卷号	409 页码:8
关键词	Molecular imprinted electrochemical sensor Boronate affinity Glycoprotein Signal amplification
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2022.135292
通讯作者	Hu, Xueping(xuephu@yeah.net) ; Song, Xingliang(xlssong@yeah.net)
英文摘要	Ovalbumin (OVA), a class of glycoproteins, is the main allergen in hen egg white that often causes allergies in humans, especially in babies. Therefore, it is pivotal to be able to detect and separate OVA. This work presents an ingenious sandwich-structured magnetic molecular imprinted electrochemical sensor for OVA detection by uti-lizing boronate affinity and signal amplification strategy. With anti-OVA antibody-modified gold nanoparticles (AuNPs) as amplifiers, the imprinted cavities in the probe could capture protein to form a sandwich structure. Due to its specific recognition of antibody and molecular imprinted polymers and the signal amplification of AuNPs, the sensor had good selectivity and sensitivity toward OVA and a low detection limit of 3.0 fg/mL. The sensor also had excellent stability and could satisfactorily detect OVA in real red wine samples.
WOS关键词	S-OVALBUMIN ; GLYCOSYLATION ; NANOPARTICLES
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
WOS记录号	WOS:000915044100001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Key Research and Development Program of Linyi City
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/32573]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Hu, Xueping; Song, Xingliang
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China
推荐引用方式 GB/T 7714	Fu, Hao,Bai, Zhenyu,Li, Ping,et al. Molecular imprinted electrochemical sensor for ovalbumin detection based on boronate affinity and signal amplification approach[J]. FOOD CHEMISTRY,2023,409:8.
APA	Fu, Hao.,Bai, Zhenyu.,Li, Ping.,Feng, Xiaoyang.,Hu, Xueping.,...&Chen, Lingxin.(2023).Molecular imprinted electrochemical sensor for ovalbumin detection based on boronate affinity and signal amplification approach.FOOD CHEMISTRY,409,8.
MLA	Fu, Hao,et al."Molecular imprinted electrochemical sensor for ovalbumin detection based on boronate affinity and signal amplification approach".FOOD CHEMISTRY 409(2023):8.