Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum

doi:10.1016/j.fsi.2022.09.068

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中国科学院牟平海岸带环境综合试验站

	Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum
	Lv, Xiaojing 1,5,6; Jiao, Chunli 4; Chen, Yuying 2; Chen, Lizhu 2; Li, Xuan 2; Liu, Xiangquan 2; Yang, Dinglong 3,5,6; Zhao, Jianmin 3,5,6
刊名	FISH & SHELLFISH IMMUNOLOGY
	2022-12-01
卷号	131 页码:77-83
关键词	Ruditapes philippinarum Histone Antimicrobial activity Extracellular traps
ISSN号	1050-4648
DOI	10.1016/j.fsi.2022.09.068
英文摘要	In the present study, a histone H2A (designed as RpH2A) was identified and characterized from clam Ruditapes philippinarum, and its open reading frame (ORF) was of 387 bp encoding a polypeptide of 128 amino acids. The deduced amino acid sequence of RpH2A shared high identities ranging from 57.1% to 96.1% with that of other identified H2A. The mRNA expression of RpH2A was up-regulated significantly after Vibrio anguillarum chal-lenge. The recombinant RpH2A protein (rRpH2A) displayed significantly binding affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN) in vitro, and also exhibited antimicrobial properties against Escherichia coli. In addition, the antimicrobial RpH2A was shown to co-localize with extracellular traps (ETs) released from he-mocytes induced by E. coli, suggesting that RpH2A might contribute to eliminate invading bacteria in clam ETs. Altogether, our data revealed that RpH2A could function as antimicrobial peptides, which might play a crucial role in the immune responses of hemocytes ETs in clams.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050303] ; National Natural Science Foundation of China[41806196] ; National Natural Science Foundation of China[42176141] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2022211] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Yantai Science and Technology Development Project[2020MSGY066]
WOS关键词	MOLECULAR CHARACTERIZATION ; DISEASE VIRUS ; EXPRESSION ; HEMOCYTES ; IMMUNITY ; PROTEIN ; ANTIBODY ; H1
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000876937200001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31850]
专题	中国科学院牟平海岸带环境综合试验站
通讯作者	Yang, Dinglong
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Shandong Marine Resources & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 4.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China 6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xiaojing,Jiao, Chunli,Chen, Yuying,et al. Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2022,131:77-83.
APA	Lv, Xiaojing.,Jiao, Chunli.,Chen, Yuying.,Chen, Lizhu.,Li, Xuan.,...&Zhao, Jianmin.(2022).Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,131,77-83.
MLA	Lv, Xiaojing,et al."Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 131(2022):77-83.