Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction

doi:10.1016/j.snb.2022.132749

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	Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction
	Zhou, Yanan 1,2,3,4; Zeng, Yan 1,2; Zhang, Dun 1,2; Qi, Peng 1,2; Wang, Yingwen 1,2,3,4; Xin, Yue 1,2,3,4; Sun, Yan 1,2
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2023
卷号	374 页码:7
关键词	Bacterial DNA DNA silver nanocluster Three-way DNA junction
DOI	10.1016/j.snb.2022.132749
通讯作者	Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要	We have constructed a simple, enzyme-free and label-free detection method for bacterial DNA. First, bacterial DNA (T) is designed as a trigger to make the designed metastable hairpin probes P1, P2, P3 (carrying DNA silver nanoclusters sequences) autonomously cross-open to achieve catalytic self-assembly, inducing a three-way DNA junction formation through the catalyzed hairpin self-assembly (CHA) mode. Then, G-rich sequences are intro-duced to form DNA silver nanoclusters G quadruplex (DNA-AgNCs-G quadruplex), and a strong fluorescence signal can be collected under the action of silver nitrate and sodium borohydride solutions. Through the fluo-rescent reporter of DNA silver nanoclusters (DNA-AgNCs) and the amplification of CHA, we successfully detected target DNA at concentrations as low as 19.95 fM. Compared with the constructed acyclic system, the sensitivity of this cyclic system is improved about three orders of magnitude. Moreover, this recycling system achieves good selectivity for mismatched target DNA and excellent recovery rate in practical samples. Therefore, bacterial DNA analysis based on target-aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction is successfully developed, promising its great application in biological sensing.
资助项目	National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Nantong Scientific Plan Foundation[JC2020090] ; Nantong Scientific Plan Foundation[JC2021054] ; Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF01] ; Shandong Provincial Marine Science and Technology Projects
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000882029800004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180280]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Zeng, Yan; Zhang, Dun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yanan,Zeng, Yan,Zhang, Dun,et al. Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,374:7.
APA	Zhou, Yanan.,Zeng, Yan.,Zhang, Dun.,Qi, Peng.,Wang, Yingwen.,...&Sun, Yan.(2023).Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction.SENSORS AND ACTUATORS B-CHEMICAL,374,7.
MLA	Zhou, Yanan,et al."Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction".SENSORS AND ACTUATORS B-CHEMICAL 374(2023):7.