Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance

doi:10.1111/mec.16765

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance
	Sun, Yan 1,2,3,4; Wang, Minxiao 1,2,3,4,6,7; Chen, Hao 1,2,3,4; Wang, Hao 1,2,3,4; Zhong, Zhaoshan 1,2,3,4; Zhou, Li 1,2,3,4; Fu, Lulu 1,2,3,4; Li, Chaolun 1,2,3,4,5; Sun, Song 1,2,3,4,5,6,7
刊名	MOLECULAR ECOLOGY
	2022-11-17
页码	16
关键词	Bathymodiolinae mussel deep-sea endosymbioses gene network analysis laboratory maintenance metatranscriptome symbiotic interaction
ISSN号	0962-1083
DOI	10.1111/mec.16765
通讯作者	Wang, Minxiao(wangminxiao@qdio.ac.cn) ; Sun, Song(sunsong@qdio.ac.cn)
英文摘要	Symbioses between invertebrates and chemosynthetic bacteria are of fundamental importance in deep-sea ecosystems, but the mechanisms that enable their symbiont associations are still largely undescribed, owing to the culturable difficulties of deep-sea lives. Bathymodiolinae mussels are remarkable in their ability to overcome decompression and can be maintained successfully for an extended period under atmospheric pressure, thus providing a model for investigating the molecular basis of symbiotic interactions. Herein, we conducted metatranscriptome sequencing and gene co-expression network analysis of Gigantidas platifrons under laboratory maintenance with gradual loss of symbionts. The results revealed that one-day short-term maintenance triggered global transcriptional perturbation in symbionts, but little gene expression changes in mussel hosts, which were mainly involved in responses to environmental changes. Long-term maintenance with depleted symbionts induced a metabolic shift in the mussel host. The most notable changes were the suppression of sterol biosynthesis and the complementary activation of terpenoid backbone synthesis in response to the reduction of bacteria-derived terpenoid sources. In addition, we detected the upregulation of host proteasomes responsible for amino acid deprivation caused by symbiont depletion. Additionally, a significant correlation between host microtubule motor activity and symbiont abundance was revealed, suggesting the possible function of microtubule-based intracellular trafficking in the nutritional interaction of symbiosis. Overall, by analyzing the dynamic transcriptomic changes during the loss of symbionts, our study highlights the nutritional importance of symbionts in supplementing terpenoid compounds and essential amino acids and provides insight into the molecular mechanisms and strategies underlying the symbiotic interactions in deep-sea ecosystems.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; National Natural Science Foundation of China ; Mount Tai Scholar Climbing Plan ; [2022QNLM030004] ; [41806200] ; [42076091] ; [42030407] ; [41906124]
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000888413200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180341]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Wang, Minxiao; Sun, Song
作者单位	1.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 7.Inst Oceanol, Chinese Acad Sci, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yan,Wang, Minxiao,Chen, Hao,et al. Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance[J]. MOLECULAR ECOLOGY,2022:16.
APA	Sun, Yan.,Wang, Minxiao.,Chen, Hao.,Wang, Hao.,Zhong, Zhaoshan.,...&Sun, Song.(2022).Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance.MOLECULAR ECOLOGY,16.
MLA	Sun, Yan,et al."Insights into symbiotic interactions from metatranscriptome analysis of deep-sea mussel Gigantidas platifrons under long-term laboratory maintenance".MOLECULAR ECOLOGY (2022):16.