Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis

doi:10.18632/aging.204242

	Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis
	Bai, Rui 1,2,3; Shi, Zhong 4; Li, Dan 1; Zhou, Donger 5; Ge, Wei-Ting 1; Zheng, Shu 1
刊名	AGING-US
	2022-08-31
卷号	14
关键词	colorectal cancer liver metastasis NKTR proliferation migration and invasion
ISSN号	1945-4589
DOI	10.18632/aging.204242
通讯作者	Zheng, Shu(zhengshu@zju.edu.cn)
英文摘要	Objective: Liver metastasis is one of the prognostic factors of colorectal cancer (CRC). The aim of this study is to identify biomarkers that facilitate easier detection of liver metastasis. Methods: Significance Analysis of Microarrays (SAM) and Array Data Analyzer (ADA) were applied used for the analysis of differentially differently expressed mRNAs. mRNA expression was verified by quantitative real-timer reverse transcriptiontase polymerase chain reaction (qRT-PCR). Immunohistochemistry were was used to show natural killer-tumor recognition (NKTR) expression in CRC. NKTR-knockdown CRC cells were constructed obtained by using short hairpin RNA (shRNA). Followed by CCK-8 assay, plate colony formation test, and transwell assay were used to evaluate the influence of NKTR on cell proliferation, migration, and invasion in vitro. Results: SAM yielded showed 256 up-regulated and 224 down-regulated differentially differently expressed genes. Seven genes were identified by using ADA, tools and four genes were verified by using qRT-PCR. Three genes (metastasis associated lung adenocarcinoma transcript 1 (MALAT1), nuclear factor I/B (NKTR), and nuclear factor I/B (NFIB)) showed a statistically significant considerably difference between CRC with and liver metastasis and CRC without liver metastasis. Immunohistochemical analysis showed that NKTR expression was much lower in primary CRC with liver metastasis than that in primary CRC without liver metastasis. The NKTR protein plays a role in the lytic function of natural killer (NK) cells and it has been rarely studied in the CRC. The down-regulation of NKTR by shRNA interference in CRC cells increased cell proliferation, migration, and invasion in vitro.
资助项目	Zhejiang Basic Public Welfare Research Project[LGF18H160040]
WOS关键词	CYCLOPHILIN ; STATISTICS ; RESECTION
WOS研究方向	Cell Biology ; Geriatrics & Gerontology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000877374200018
资助机构	Zhejiang Basic Public Welfare Research Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131685]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Shu
作者单位	1.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Canc Inst,Key Lab Canc Prevent & Intervent,China, Hangzhou 310002, Zhejiang, Peoples R China 2.Zhejiang Univ, Affiliated Hosp 2, Dept Med Oncol, Key Lab Canc Prevent & Intervent,Minist Educ,Sch, Hangzhou 310009, Zhejiang, Peoples R China 3.Zhejiang Univ, Canc Ctr, Hangzhou 310058, Zhejiang, Peoples R China 4.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Canc & Basic Med ICBM, Dept Med Oncol,Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 5.Zhejiang Univ, Affiliated Hosp 2, Dept Surg, Sch Med, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Bai, Rui,Shi, Zhong,Li, Dan,et al. Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis[J]. AGING-US,2022,14.
APA	Bai, Rui,Shi, Zhong,Li, Dan,Zhou, Donger,Ge, Wei-Ting,&Zheng, Shu.(2022).Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis.AGING-US,14.
MLA	Bai, Rui,et al."Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis".AGING-US 14(2022).