Total flavones from Sceptridium ternatum alleviate pulmonary hypertension through inhibiting the proliferation of vascular endothelial cells

doi:10.21037/atm-21-5889

	Total flavones from Sceptridium ternatum alleviate pulmonary hypertension through inhibiting the proliferation of vascular endothelial cells
	Li, Qinglin 1; Xin, Wenxiu 1; Ding, Haiyin 1; Zhang, Yiwen 2,3; Zheng, Xiaowei 2,3; Liu, Yujia 2; Huang, Ping 2,3
刊名	ANNALS OF TRANSLATIONAL MEDICINE
	2022-05-27
关键词	Total flavones pulmonary hypertension (PH) HIF-? JAK2-STAT3 Sceptridium ternatum
ISSN号	2305-5839
DOI	10.21037/atm-21-5889
通讯作者	Huang, Ping(huangping1841@zjcc.org.cn)
英文摘要	Background: Sceptridium ternatum is a traditional Chinese medicine that is prescribed to treat respiratory diseases in China. Our previous study confirmed that total flavones from Sceptridium ternatum (FST) have preventive and therapeutic effects on pulmonary hypertension (PH). The present study sought to investigate the mechanism underpinning the therapeutic efficacy of FST in PH. Methods: Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, real-time quantitative PCR (RT-qPCR), western blot, flow cytometry, high-throughput sequencing, and bioinformatics analysis were performed to study the therapeutic mechanism of FST in PH at the gene, cell, and animal levels. Results: The results showed that FST could inhibit the proliferation of both human pulmonary artery smooth muscle cells (HPASMCs) and human pulmonary microvascular endothelial cells (HPMECs), and downregulate the expression of HIF1?? and HIF2??, which are the key factors in the pathogenesis and occurrence of PH. FST could also inhibit the activation of the downstream JAK2-STAT3 signaling pathway and upregulate the expression of the negative regulator SOCS1. Vascular endothelial cell and smooth muscle cell proliferation was inhibited and the symptoms of PH were relieved by FST. Conclusions: The findings of this study offer important clues for the identification of new molecular targets in FST treatment and the development of treatment strategies for PH.
资助项目	Zhejiang Natural Science Foundation[LQ19H28001] ; National Natural Science Foundation of China[81903898] ; Zhejiang Province Traditional Chinese Medicine Key Scientific Research Fund Project[2016ZZ009] ; Zhejiang Province Traditional Chinese Medicine Key Scientific Research Fund Project[2018ZZ006] ; Zhejiang Province Traditional Chinese Medicine Key Scientific Research Fund Project[2021ZZ001] ; Zhejiang Province Traditional Chinese Medicine Key Scientific Research Fund Project[2022ZZ001] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2016ZQ009] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2017ZB022] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2011ZQ001] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2021ZA006] ; Zhejiang Medical and Health Science and Technology Project[2016KYB061] ; Zhejiang Medical and Health Science and Technology Project[2016KYB038] ; Zhejiang Medical and Health Science and Technology Project[2016KYB033] ; Zhejiang Medical and Health Science and Technology Project[2019ZD024] ; '10000 Talents Plan' of Zhejiang Province[2020R52029] ; Zhejiang Province Health Innovation Personnel Training Program ; Second Level Training Program of Zhejiang Province's 151 Talents Project ; Zhejiang Provincial Program for the Cultivation of New Heath Talents
WOS关键词	APOPTOSIS
WOS研究方向	Oncology ; Research & Experimental Medicine
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000812176000001
资助机构	Zhejiang Natural Science Foundation ; National Natural Science Foundation of China ; Zhejiang Province Traditional Chinese Medicine Key Scientific Research Fund Project ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project ; Zhejiang Medical and Health Science and Technology Project ; '10000 Talents Plan' of Zhejiang Province ; Zhejiang Province Health Innovation Personnel Training Program ; Second Level Training Program of Zhejiang Province's 151 Talents Project ; Zhejiang Provincial Program for the Cultivation of New Heath Talents
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131359]
专题	中国科学院合肥物质科学研究院
通讯作者	Huang, Ping
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou, Peoples R China 2.Zhejiang Prov Peoples Hosp, Hangzhou Med Coll, Peoples Hosp, Clin Pharm Ctr,Dept Pharm, 1 Banshan Rd, Hangzhou 310022, Zhejiang, Peoples R China 3.Key Lab Endocrine Gland Dis Zhejiang Prov, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Li, Qinglin,Xin, Wenxiu,Ding, Haiyin,et al. Total flavones from Sceptridium ternatum alleviate pulmonary hypertension through inhibiting the proliferation of vascular endothelial cells[J]. ANNALS OF TRANSLATIONAL MEDICINE,2022.
APA	Li, Qinglin.,Xin, Wenxiu.,Ding, Haiyin.,Zhang, Yiwen.,Zheng, Xiaowei.,...&Huang, Ping.(2022).Total flavones from Sceptridium ternatum alleviate pulmonary hypertension through inhibiting the proliferation of vascular endothelial cells.ANNALS OF TRANSLATIONAL MEDICINE.
MLA	Li, Qinglin,et al."Total flavones from Sceptridium ternatum alleviate pulmonary hypertension through inhibiting the proliferation of vascular endothelial cells".ANNALS OF TRANSLATIONAL MEDICINE (2022).