Lipid profiling in malignant mesothelioma reveals promising signatures for diagnosis and prognosis: A plasma-based LC-MS lipidomics study

doi:10.1016/j.cca.2021.11.024

	Lipid profiling in malignant mesothelioma reveals promising signatures for diagnosis and prognosis: A plasma-based LC-MS lipidomics study
	Chen, Zhongjian ; Song, Siyu ; Yang, Chenxi ; Dai, Ziyi ; Gao, Yun ; Li, Na ; Zhu, Jing ; Mao, Weimin 1,2; Liu, Junping 1,2
刊名	CLINICA CHIMICA ACTA
	2022
卷号	524
关键词	LC-MS Malignant mesothelioma Lipidomics TG PC PE Ceramide Biomarker
ISSN号	0009-8981
DOI	10.1016/j.cca.2021.11.024
通讯作者	Mao, Weimin(maowm@zjcc.org.cn) ; Liu, Junping(liujunpinzjcc@163.com)
英文摘要	Background and aim: Malignant mesothelioma (MM), being a rare and aggressive carcinoma, can barely be cured. Incidence of this cancer will keep climbing up in the next few decades since its major carcinogen, asbestos, is still in use in many countries. Unfortunately, prognosis of MM is unsatisfactory principally due to poor early diag-nosis as a result of its long latency period and ambiguous symptoms. Lipids are known to contribute to cellular structure, signaling, and energy storage, and are widely reported to be related with tumorigenesis. Therefore, we aim to discover novel lipid biomarkers by plasma-based lipidomics that may improve MM diagnosis. Methods: Plasma samples from 25 MM patients and 32 healthy controls (HCs) were collected and analysed using a high-throughput liquid chromatography-mass spectrometry (LC-MS). Univariate and multivariate analyses were subsequently performed to visualize the separation trend between two groups and to screen for differential feature ions. Ions were annotated using LipidSearch 4.2 and their enriched pathways were detected on LIPEA. Receiver operating characteristic (ROC) curves were used for analysing each annotated lipid's diagnostic value. Survival analyses were performed to investigate each lipid's prognostic value. Results: In supervised partial least squares discriminant analysis (PLS-DA), clear separation between MM and HC groups was observed. A total of 34 differential lipids were annotated, among which 5 upregulated and 29 downregulated. Levels of plasma triacylglycerols (TGs) were higher in smoking versus non-smoking patients, and lower in female versus male patients. The top six lipids possessing highest diagnostic value included two phosphatidylethanolamines (PEs), two phosphatidylcholines (PCs) and two ceramides. Moreover, elevated circulating TG levels were associated with poorer survival, whereas increased monohexosylceramide (Hex1Cer) might be beneficial. Conclusions: Our study revealed differentially expressed lipid patterns in MM compared to HC. PC, PE, and ceramides showed outstanding diagnostic performance, while TG and Hex1Cer exhibited significant prognostic values. Nevertheless, more studies should verify these trends as well as further investigating on underlying mechanisms.
资助项目	Key R&D Program Projects in Zhejiang Province[2018C04009] ; National Natural Science Foundation of China[81672315] ; National Natural Science Foundation of China[82072577] ; International Cooperation Project of Zhejiang Basic Public Technology Research Program[LGJ20H010001] ; Projects of Zhejiang Province Medical and Health Science and Technology Plan[2017KY256] ; Projects of Zhejiang Province Medical and Health Science and Technology Plan[2022KY622]
WOS关键词	CANCER
WOS研究方向	Medical Laboratory Technology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000790634000005
资助机构	Key R&D Program Projects in Zhejiang Province ; National Natural Science Foundation of China ; International Cooperation Project of Zhejiang Basic Public Technology Research Program ; Projects of Zhejiang Province Medical and Health Science and Technology Plan
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130861]
专题	中国科学院合肥物质科学研究院
通讯作者	Mao, Weimin; Liu, Junping
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 2.Zhejiang Key Lab Diag & Treatment Technol Thorac, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Zhongjian,Song, Siyu,Yang, Chenxi,et al. Lipid profiling in malignant mesothelioma reveals promising signatures for diagnosis and prognosis: A plasma-based LC-MS lipidomics study[J]. CLINICA CHIMICA ACTA,2022,524.
APA	Chen, Zhongjian.,Song, Siyu.,Yang, Chenxi.,Dai, Ziyi.,Gao, Yun.,...&Liu, Junping.(2022).Lipid profiling in malignant mesothelioma reveals promising signatures for diagnosis and prognosis: A plasma-based LC-MS lipidomics study.CLINICA CHIMICA ACTA,524.
MLA	Chen, Zhongjian,et al."Lipid profiling in malignant mesothelioma reveals promising signatures for diagnosis and prognosis: A plasma-based LC-MS lipidomics study".CLINICA CHIMICA ACTA 524(2022).